痘苗病毒导向的脱氧核糖核酸聚合酶的研究
Studies on vaccinia virus-directed deoxyribonucleic acid polymerase.
作者信息
Citarella R V, Muller R, Schlabach A, Weissbach A
出版信息
J Virol. 1972 Oct;10(4):721-9. doi: 10.1128/JVI.10.4.721-729.1972.
Abstract
A vaccinia-directed deoxyribonucleic acid (DNA) polymerase has been partially purified from the cytoplasmic fractions of virus-infected HeLa cells. The utilization of natural and synthetic templates by this enzyme resembles that of the host cell DNA-dependent DNA polymerases. The vaccinia DNA polymerase cannot copy ribopolymers or ribonucleic acid but is very effective with an "activated" DNA as template. An exonuclease preferring single-stranded DNA as substrate is found in the most highly purified preparations of the enzyme. The molecular weight of the vaccinia DNA polymerase seems to be about 110,000. The viral DNA polymerase is also found to be associated with purified, infected cell nuclei, and this association may be due, at least in part, to nonspecific adsorption of the vaccinia DNA polymerase by nuclei.
摘要
已从病毒感染的HeLa细胞的细胞质部分中部分纯化出一种痘苗导向的脱氧核糖核酸（DNA）聚合酶。该酶对天然和合成模板的利用类似于宿主细胞依赖DNA的DNA聚合酶。痘苗DNA聚合酶不能复制核糖聚合物或核糖核酸，但以“活化”DNA为模板时非常有效。在该酶的最高度纯化制剂中发现了一种以单链DNA为底物的核酸外切酶。痘苗DNA聚合酶的分子量似乎约为110,000。还发现病毒DNA聚合酶与纯化的感染细胞核相关联，这种关联可能至少部分是由于痘苗DNA聚合酶被细胞核非特异性吸附所致。
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本文引用的文献
1
2
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J Biol Chem. 1963 Oct;238:3383-9.
3
Separation of a new deoxyribonucleic acid polymerase from vaccinia-infected HeLa cells.从感染痘苗病毒的海拉细胞中分离出一种新的脱氧核糖核酸聚合酶。
J Virol. 1969 Jul;4(1):15-23. doi: 10.1128/JVI.4.1.15-23.1969.
4
A simple and rapid method for the isolation of enzymatically active HeLa cell nuclei.一种分离具有酶活性的海拉细胞核的简单快速方法。
J Cell Biol. 1969 Sep;42(3):851-4. doi: 10.1083/jcb.42.3.851.
5
DNA polymerases from human cells.来自人类细胞的DNA聚合酶。
Nat New Biol. 1971 Jun 9;231(23):167-70. doi: 10.1038/newbio231167a0.
6
DNA-dependent DNA polymerases from HeLa cell nuclei. II. Template and substrate utilization.来自海拉细胞核的依赖DNA的DNA聚合酶。II. 模板和底物的利用
Biochem Biophys Res Commun. 1971 Aug 20;44(4):879-85. doi: 10.1016/0006-291x(71)90793-5.
7
8
A new synthetic RNA-dependent DNA polymerase from human tissue culture cells (HeLa-fibroblast-synthetic oligonucleotides-template-purified enzymes).一种来自人组织培养细胞的新型合成RNA依赖性DNA聚合酶（海拉细胞-成纤维细胞-合成寡核苷酸-模板-纯化酶）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):452-5. doi: 10.1073/pnas.69.2.452.
9
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10
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