来自KB细胞的信使核糖核蛋白和信使核糖核酸的特性分析。
Characterization of messenger ribonucleoprotein and messenger RNA from KB cells.
作者信息
Kumar A, Lindberg U
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):681-5. doi: 10.1073/pnas.69.3.681.
Abstract
Messenger ribonucleoprotein and mRNA from KB-cells were isolated under conditions designed to minimize nonspecific RNA-protein interaction and to minimize degradation by contaminating ribonucleases. A large fraction, 60-70%, of the messenger ribonucleoprotein from polysomes dissociated in vitro by either EDTA or puromycin sedimented faster than the large ribosome subunit. Messenger ribonucleoprotein particles with sedimentation coefficients up to 200 S were observed. Released mRNA was also large, with maximal molecular weights around 5 x 10(6).
摘要
在旨在尽量减少非特异性RNA - 蛋白质相互作用并尽量减少污染核糖核酸酶降解的条件下，从KB细胞中分离出信使核糖核蛋白和mRNA。通过EDTA或嘌呤霉素在体外解离的多核糖体中的大部分信使核糖核蛋白（60 - 70%）沉降速度比大核糖体亚基快。观察到沉降系数高达200 S的信使核糖核蛋白颗粒。释放出的mRNA也很大，最大分子量约为5×10⁶ 。
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