乳糖阻遏蛋白对非操纵基因DNA小沟的优先保护作用，使其免受硫酸二甲酯甲基化修饰。
Preferential protection of the minor groove of non-operator DNA by lac repressor against methylation by dimethyl sulphate.
作者信息
Kolchinsky A M, Mirzabekov A D, Gilbert W, Li L
出版信息
Nucleic Acids Res. 1976 Jan;3(1):11-8. doi: 10.1093/nar/3.1.11.
Abstract
The binding of lactose repressor to non-operator DNA was studied by the modification of several DNA's, including glycosylated DNA, with dimethyl sulphate, which affects the minor and major grooves of DNA and single stranded DNA regions. The non-specific binding of the repressor to DNA protected the minor groove but apparently not the major groove of the DNA double helix against methylation and did not increase the content of single stranded DNA regions. This suggests that the repressor on binding to non-operator DNA makes contacts mainly in the minor groove of DNA and does not uncoil the DNA double helix. This is different from the interaction of the repressor with lactose operator DNA which occurs, as shown by Gilbert et al. (1), along both the major and the minor groove.
摘要
通过用硫酸二甲酯修饰包括糖基化DNA在内的几种DNA，研究了乳糖阻遏物与非操纵基因DNA的结合情况。硫酸二甲酯会影响DNA的小沟和大沟以及单链DNA区域。阻遏物与DNA的非特异性结合保护了DNA双螺旋的小沟免受甲基化影响，但显然没有保护大沟，并且没有增加单链DNA区域的含量。这表明阻遏物在与非操纵基因DNA结合时主要在DNA的小沟中形成接触，并且不会使DNA双螺旋解旋。这与阻遏物与乳糖操纵基因DNA的相互作用不同，如吉尔伯特等人（1）所示，后者沿着大沟和小沟都有发生。
相似文献
1
Preferential protection of the minor groove of non-operator DNA by lac repressor against methylation by dimethyl sulphate.乳糖阻遏蛋白对非操纵基因DNA小沟的优先保护作用，使其免受硫酸二甲酯甲基化修饰。
Nucleic Acids Res. 1976 Jan;3(1):11-8. doi: 10.1093/nar/3.1.11.
2
The arginine repressor of Escherichia coli K-12 makes direct contacts to minor and major groove determinants of the operators.大肠杆菌K-12的精氨酸阻遏物与操纵子的小沟和大沟决定簇直接接触。
J Mol Biol. 1998 Apr 10;277(4):805-24. doi: 10.1006/jmbi.1998.1632.
3
Interaction of lambda cro repressor with synthetic operator OR3 studied by competition binding with minor groove binders.通过与小沟结合剂的竞争结合研究λ cro阻遏蛋白与合成操纵子OR3的相互作用。
J Biomol Struct Dyn. 1992 Aug;10(1):15-33. doi: 10.1080/07391102.1992.10508627.
4
Sites of contact between lambda operators and lambda repressor.λ 操纵基因与 λ 阻遏物之间的接触位点。
Nucleic Acids Res. 1977;4(5):1595-607. doi: 10.1093/nar/4.5.1595.
5
The role of lysine 55 in determining the specificity of the purine repressor for its operators through minor groove interactions.赖氨酸55通过小沟相互作用在决定嘌呤阻遏物对其操纵基因的特异性方面所起的作用。
J Mol Biol. 1999 Aug 13;291(2):347-61. doi: 10.1006/jmbi.1999.2946.
6
Binding of E.coli lac repressor to non-operator DNA.大肠杆菌乳糖阻遏物与非操纵基因DNA的结合。
Nucleic Acids Res. 1977;4(5):1579-93. doi: 10.1093/nar/4.5.1579.
7
Transcription regulation in thermophilic bacteria: high resolution contact probing of Bacillus stearothermophilus and Thermotoga neapolitana arginine repressor-operator interactions.嗜热细菌中的转录调控：嗜热脂肪芽孢杆菌和那不勒斯嗜热栖热菌精氨酸阻遏物-操纵基因相互作用的高分辨率接触探测
J Mol Biol. 2002 Jan 18;315(3):255-74. doi: 10.1006/jmbi.2001.5236.
8
A study of unwinding of DNA and shielding of the DNA grooves by RNA polymerase by using methylation with dimethylsulphate.
Eur J Biochem. 1978 Mar;84(1):301-9. doi: 10.1111/j.1432-1033.1978.tb12169.x.
9
A model for the binding of lac repressor to the lac operator.一种乳糖阻遏物与乳糖操纵基因结合的模型。
Mol Biol Rep. 1976 Apr;2(5):427-34. doi: 10.1007/BF00366265.
10
Plasticity in protein-DNA recognition: lac repressor interacts with its natural operator 01 through alternative conformations of its DNA-binding domain.蛋白质 - DNA 识别中的可塑性：乳糖阻遏物通过其 DNA 结合结构域的不同构象与天然操纵子 O1 相互作用。
EMBO J. 2002 Jun 17;21(12):2866-76. doi: 10.1093/emboj/cdf318.
引用本文的文献
1
Model for lactose repressor protein and its interaction with ligands.乳糖阻遏蛋白模型及其与配体的相互作用。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7181-5. doi: 10.1073/pnas.77.12.7181.
2
lac repressor changes conformation upon binding to poly[dA-T)].乳糖阻遏蛋白在与聚[dA-T]结合后会改变构象。
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2649-53. doi: 10.1073/pnas.76.6.2649.
3
Binding of E.coli lac repressor to non-operator DNA.大肠杆菌乳糖阻遏物与非操纵基因DNA的结合。
Nucleic Acids Res. 1977;4(5):1579-93. doi: 10.1093/nar/4.5.1579.
4
Contacts between the lac repressor and the thymines in the lac operator.乳糖阻遏蛋白与乳糖操纵基因中胸腺嘧啶之间的相互作用。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4973-6. doi: 10.1073/pnas.74.11.4973.
5
Secondary structural complementarity between DNA and proteins.DNA与蛋白质之间的二级结构互补性。
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1458-62. doi: 10.1073/pnas.74.4.1458.
本文引用的文献
1
Isolation of the lac repressor.乳糖阻遏蛋白的分离
Proc Natl Acad Sci U S A. 1966 Dec;56(6):1891-8. doi: 10.1073/pnas.56.6.1891.
2
FURTHER STUDIES ON THE ALKYLATION OF NUCLEIC ACIDS AND THEIR CONSTITUENT NUCLEOTIDES.核酸及其组成核苷酸烷基化的进一步研究
Biochem J. 1963 Oct;89(1):127-38. doi: 10.1042/bj0890127.
3
Lac repressor binding to operator analogues: comparison of poly(d(A-T)), poly(d(A-BrU)), and poly(d(A-U)).乳糖阻遏蛋白与操纵基因类似物的结合：聚（d（A-T））、聚（d（A-BrU））和聚（d（A-U））的比较
Biochem Biophys Res Commun. 1971 Dec 17;45(6):1542-7. doi: 10.1016/0006-291x(71)90195-1.
4
Studies with glucosyl transferase mutants of the T-even bacteriophages.T偶数噬菌体葡萄糖基转移酶突变体的研究。
Virology. 1971 May;44(2):271-85. doi: 10.1016/0042-6822(71)90259-5.
5
The lac repressor-operator interaction. 3. Kinetic studies.乳糖阻遏蛋白与操纵基因的相互作用。3. 动力学研究。
J Mol Biol. 1970 Nov 14;53(3):401-17. doi: 10.1016/0022-2836(70)90074-4.
6
Lac repressor binding to non-operator DNA: detailed studies and a comparison of eequilibrium and rate competition methods.乳糖阻遏蛋白与非操纵基因DNA的结合：详细研究以及平衡和速率竞争方法的比较
J Mol Biol. 1972 Dec 30;72(3):671-90. doi: 10.1016/0022-2836(72)90184-2.
7
Lac repressor binding to synthetic DNAs of defined nucleotide sequence.乳糖阻遏蛋白与特定核苷酸序列的合成DNA的结合。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):761-4. doi: 10.1073/pnas.69.3.761.
8
Lac repressor binding to poly (d(A-T)). Conformational changes.乳糖阻遏蛋白与聚（d（A-T））的结合。构象变化。
Biochem Biophys Res Commun. 1974 Oct 8;60(3):951-7. doi: 10.1016/0006-291x(74)90406-9.
9
10
The general affinity of lac repressor for E. coli DNA: implications for gene regulation in procaryotes and eucaryotes.乳糖阻遏蛋白对大肠杆菌DNA的总体亲和力：对原核生物和真核生物基因调控的意义
Cell. 1975 Feb;4(2):107-11. doi: 10.1016/0092-8674(75)90116-6.
Zotero 插件