Kolchinsky A M, Mirzabekov A D, Gilbert W, Li L
Nucleic Acids Res. 1976 Jan;3(1):11-8. doi: 10.1093/nar/3.1.11.
The binding of lactose repressor to non-operator DNA was studied by the modification of several DNA's, including glycosylated DNA, with dimethyl sulphate, which affects the minor and major grooves of DNA and single stranded DNA regions. The non-specific binding of the repressor to DNA protected the minor groove but apparently not the major groove of the DNA double helix against methylation and did not increase the content of single stranded DNA regions. This suggests that the repressor on binding to non-operator DNA makes contacts mainly in the minor groove of DNA and does not uncoil the DNA double helix. This is different from the interaction of the repressor with lactose operator DNA which occurs, as shown by Gilbert et al. (1), along both the major and the minor groove.
通过用硫酸二甲酯修饰包括糖基化DNA在内的几种DNA，研究了乳糖阻遏物与非操纵基因DNA的结合情况。硫酸二甲酯会影响DNA的小沟和大沟以及单链DNA区域。阻遏物与DNA的非特异性结合保护了DNA双螺旋的小沟免受甲基化影响，但显然没有保护大沟，并且没有增加单链DNA区域的含量。这表明阻遏物在与非操纵基因DNA结合时主要在DNA的小沟中形成接触，并且不会使DNA双螺旋解旋。这与阻遏物与乳糖操纵基因DNA的相互作用不同，如吉尔伯特等人（1）所示，后者沿着大沟和小沟都有发生。
