兔网织红细胞核糖体后上清液中的珠蛋白信使核糖核酸。
Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.
作者信息
Jacobs-Lorena M, Baglioni C
出版信息
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1425-8. doi: 10.1073/pnas.69.6.1425.
Abstract
10S RNA has been isolated from the postribosomal supernatant of rabbit reticulocyte lysate. This RNA is associated with protein in a ribonucleoprotein particle that sediments at approximately 20S. The 10S RNA extracted from this particle codes predominantly for alpha globin chains when it is translated by a mouse ascites cell-free extract. The product of the cell-free synthesis has been characterized by column chromatography and electrophoresis of the tryptic peptides. About 20% of the total alpha chain mRNA is present in the postribosomal supernatant, whereas beta chain mRNA is almost exclusively associated with polyribosomes.
摘要
10S RNA已从兔网织红细胞裂解物的核糖体后上清液中分离出来。这种RNA与一种沉降系数约为20S的核糖核蛋白颗粒中的蛋白质相关联。从该颗粒中提取的10S RNA在由小鼠腹水无细胞提取物进行翻译时，主要编码α珠蛋白链。无细胞合成产物已通过胰蛋白酶肽段的柱色谱和电泳进行了表征。约20%的α链mRNA存在于核糖体后上清液中，而β链mRNA几乎完全与多核糖体相关联。
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Proc Natl Acad Sci U S A. 1972 Jun;69(6):1425-8. doi: 10.1073/pnas.69.6.1425.
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The synthesis of mouse hemoglobin beta-chains in a rabbit reticulocyte cell-free system programmed with mouse reticulocyte 9S RNA.在用小鼠网织红细胞9S RNA编程的兔网织红细胞无细胞体系中合成小鼠血红蛋白β链。
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Hemoglobin messenger ribonucleic acid. Synthesis of 9S and ribosomal ribonucleic acid during erythroid cell development.血红蛋白信使核糖核酸。红细胞生成过程中9S和核糖体核糖核酸的合成。
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Control of haemoglobin synthesis: a difference in the size of the polysomes making alpha and beta chains.血红蛋白合成的调控：合成α链和β链的多核糖体大小存在差异。
Nature. 1968 Nov 2;220(5166):481-3. doi: 10.1038/220481a0.
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