Whitmore D, Whitmore E, Gilbert L I
Proc Natl Acad Sci U S A. 1972 Jun;69(6):1592-5. doi: 10.1073/pnas.69.6.1592.
Within a few hours after injection of juvenile hormone into Hyalophora gloveri pupae, several fast-migrating carboxylesterases (EC 3.1.1.1) that are sensitive to diisopropylfluorophosphate appear in the hemolymph. Treatment of the pupae with puromycin or actinomycin D prevents the appearance of these hemolymph enzymes, suggesting de novo synthesis of the carboxylesterases. Of the several other compounds investigated, only a potent mimic of the juvenile hormone is able to induce these enzymes. When the induced enzymes are incubated in vitro with (14)C-labeled juvenile hormone, the hormone is rapidly and efficiently degraded. It is suggested that these induced carboxylesterases play an important role in the regulation of juvenile hormone titer.
在将保幼激素注射到格氏柞蚕蛹内的几小时内，血淋巴中会出现几种对二异丙基氟磷酸敏感的快速迁移的羧酸酯酶（EC 3.1.1.1）。用嘌呤霉素或放线菌素D处理蛹可阻止这些血淋巴酶的出现，这表明羧酸酯酶是重新合成的。在研究的其他几种化合物中，只有一种强效的保幼激素类似物能够诱导这些酶。当将诱导产生的酶与（14）C标记的保幼激素在体外孵育时，该激素会迅速且高效地降解。有人认为，这些诱导产生的羧酸酯酶在保幼激素滴度的调节中起重要作用。
