黑腹果蝇早期发育过程中DNA聚合酶活性的研究
Survey of DNA polymerase activity during the early development of Drosophila melanogaster.
作者信息
Margulies L, Chargaff E
出版信息
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2946-50. doi: 10.1073/pnas.70.10.2946.
Abstract
The pattern of DNA polymerase activity in developing Drosophila melanogaster has been studied in seven stages of embryonic development as well as in unfertilized eggs. The crude polymerase-containing extracts, most likely of cytoplasmic origin, utilize, in the following order of decreasing template efficiency, "activated" calfthymus DNA, poly(A).oligo(dT), and poly(A).oligo(U). The highest enzymes levels occur in unfertilized eggs; the activity remains high during the first 9 hr of embryogenesis, but shows a progressive decline in the later stages. Deoxyribonuclease exhibits a similar trend. The unfertilized eggs of two genotypically different females had nearly identical levels of DNA polymerase.
摘要
在黑腹果蝇胚胎发育的七个阶段以及未受精卵中，对DNA聚合酶活性模式进行了研究。最有可能源自细胞质的含粗聚合酶提取物，按照模板效率递减的顺序利用“活化的”小牛胸腺DNA、聚（A）·寡聚（dT）和聚（A）·寡聚（U）。酶水平最高出现在未受精卵中；在胚胎发生的前9小时活性保持较高，但在后期逐渐下降。脱氧核糖核酸酶呈现出类似的趋势。两个基因型不同的雌性的未受精卵具有几乎相同水平的DNA聚合酶。
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