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Survey of DNA polymerase activity during the early development of Drosophila melanogaster.黑腹果蝇早期发育过程中DNA聚合酶活性的研究
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2946-50. doi: 10.1073/pnas.70.10.2946.
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引用本文的文献

1
The DNA polymerases of ..的DNA聚合酶
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Mutagenesis in Oocytes of DROSOPHILA MELANOGASTER. I. Scheduled Synthesis of Nuclear and Mitochondrial DNA and Unscheduled DNA Synthesis.黑腹果蝇卵母细胞的诱变。I. 核和线粒体 DNA 的定时合成与非定时 DNA 合成。
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Apurinic endonuclease activity remains constant during early Drosophila development.脱嘌呤内切酶活性在果蝇早期发育过程中保持恒定。
Cell Biol Toxicol. 1984 Oct;1(1):127-43. doi: 10.1007/BF00125570.

本文引用的文献

1
POSSIBLE SYNTHESIS OF POLYRIBONUCLEOTIDES OF KNOWN BASE-TRIPLET SEQUENCES.已知碱基三联体序列的多核糖核苷酸的可能合成
Nature. 1965 Apr 3;206:93. doi: 10.1038/206093b0.
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[Synthesis of desoxyribonucleic acid during the course of the development of Drosophila].
Biochim Biophys Acta. 1958 Aug;29(2):246-55. doi: 10.1016/0006-3002(58)90181-1.
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The relation of the heterochromatic chromosome regions to the nucleic acids of the cell.异染色质染色体区域与细胞核酸的关系。
Cold Spring Harb Symp Quant Biol. 1956;21:307-28. doi: 10.1101/sqb.1956.021.01.025.
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Deoxyribonuclease and deoxyribonucleic acid polymerase activity during Drosophila development.
Dev Biol. 1967 Jan;15(1):23-32. doi: 10.1016/0012-1606(67)90003-6.
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Sheep kidney nuclease. Hydrolysis of tRNA.羊肾核酸酶。转运核糖核酸的水解
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6
The DNA content of sperm of Drosophila melanogaster.黑腹果蝇精子的DNA含量。
Chromosoma. 1971;33(1):1-18. doi: 10.1007/BF00326379.
7
Deoxynucleotide-polymerizing enzymes of calf thymus gland. V. Homogeneous terminal deoxynucleotidyl transferase.小牛胸腺的脱氧核苷酸聚合酶。V. 均一的末端脱氧核苷酸转移酶。
J Biol Chem. 1971 Feb 25;246(4):909-16.
8
Nucleic acid polymerases of the developing chicken embryo: a DNA polymerase preferring a hybrid template.发育中的鸡胚核酸聚合酶：一种偏好杂交模板的DNA聚合酶。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2207-11. doi: 10.1073/pnas.68.9.2207.
9
DNA polymerase II.DNA聚合酶II
Nature. 1970 Dec 12;228(5276):1050-3. doi: 10.1038/2281050a0.
10
DNA polymerase of chicken embryo: purification and properties.鸡胚DNA聚合酶：纯化及性质
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1781-5. doi: 10.1073/pnas.69.7.1781.

黑腹果蝇早期发育过程中DNA聚合酶活性的研究

Survey of DNA polymerase activity during the early development of Drosophila melanogaster.

作者信息

Margulies L, Chargaff E

出版信息

Proc Natl Acad Sci U S A. 1973 Oct;70(10):2946-50. doi: 10.1073/pnas.70.10.2946.

DOI:10.1073/pnas.70.10.2946
PMID:4200726
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC427144/
Abstract

The pattern of DNA polymerase activity in developing Drosophila melanogaster has been studied in seven stages of embryonic development as well as in unfertilized eggs. The crude polymerase-containing extracts, most likely of cytoplasmic origin, utilize, in the following order of decreasing template efficiency, "activated" calfthymus DNA, poly(A).oligo(dT), and poly(A).oligo(U). The highest enzymes levels occur in unfertilized eggs; the activity remains high during the first 9 hr of embryogenesis, but shows a progressive decline in the later stages. Deoxyribonuclease exhibits a similar trend. The unfertilized eggs of two genotypically different females had nearly identical levels of DNA polymerase.

摘要

在黑腹果蝇胚胎发育的七个阶段以及未受精卵中，对DNA聚合酶活性模式进行了研究。最有可能源自细胞质的含粗聚合酶提取物，按照模板效率递减的顺序利用“活化的”小牛胸腺DNA、聚（A）·寡聚（dT）和聚（A）·寡聚（U）。酶水平最高出现在未受精卵中；在胚胎发生的前9小时活性保持较高，但在后期逐渐下降。脱氧核糖核酸酶呈现出类似的趋势。两个基因型不同的雌性的未受精卵具有几乎相同水平的DNA聚合酶。