环境pH对细胞杂交效率的影响。
Effect of environmental pH on the efficiency of cellular hybridization.
作者信息
Croce C M, Koprowski H, Eagle H
出版信息
Proc Natl Acad Sci U S A. 1972 Jul;69(7):1953-6. doi: 10.1073/pnas.69.7.1953.
Abstract
The hybridization of a human and mouse cell was strikingly pH-dependent, with a well defined optimum at (about) pH 7.6-8.0. The yield of hybrid cell colonies (1 per 500-2000 heterokaryocytes) was several hundred times greater than that obtained at pH 6.8-7.2. Although there was a significant effect on the efficiency of cell fusion, the critical time for the pH effect was in the first 4-8 days after fusion, presumably while viable hybrids were being formed from the multinucleated heterokaryocytes.
摘要
人和小鼠细胞的杂交显著依赖于pH值，在（约）pH 7.6 - 8.0时有明确的最佳值。杂交细胞集落的产量（每500 - 2000个异核细胞产生1个）比在pH 6.8 - 7.2时获得的产量高数百倍。尽管对细胞融合效率有显著影响，但pH值影响的关键时期是在融合后的前4 - 8天，大概是在多核异核细胞形成有活力的杂种细胞的时候。
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本文引用的文献
1
HYBRID CELLS DERIVED FROM MOUSE AND MAN: ARTIFICIAL HETEROKARYONS OF MAMMALIAN CELLS FROM DIFFERENT SPECIES.源自小鼠和人类的杂交细胞：不同物种哺乳动物细胞的人工异核体
Nature. 1965 Feb 13;205:640-6. doi: 10.1038/205640a0.
2
EFFECT OF HALOGENATED PYRIMIDINES AND THYMIDINE ON GROWTH OF L-CELLS AND A SUBLINE LACKING THYMIDINE KINASE.卤代嘧啶和胸腺嘧啶对L细胞及缺乏胸苷激酶的亚系细胞生长的影响
Exp Cell Res. 1964 Jan;33:19-28. doi: 10.1016/s0014-4827(64)81006-5.
3
Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
Science. 1959 Aug 21;130(3373):432-7. doi: 10.1126/science.130.3373.432.
4
Hydrogen ion buffers for biological research.用于生物学研究的氢离子缓冲剂。
Biochemistry. 1966 Feb;5(2):467-77. doi: 10.1021/bi00866a011.
5
Preliminary analysis of the requirements for fusion from within and fusion from without by Newcastle disease virus.新城疫病毒介导的体内融合和体外融合的需求初步分析
Proc Natl Acad Sci U S A. 1969 Oct;64(2):536-43. doi: 10.1073/pnas.64.2.536.
6
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Science. 1971 Oct 29;174(4008):500-3. doi: 10.1126/science.174.4008.500.
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The use of drug-resistant markers to study the hybridization of mouse fibroblasts.使用耐药标记物研究小鼠成纤维细胞的杂交。
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