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1
Structure of the radical form of clostridial flavodoxin: a new molecular model.梭菌黄素氧还蛋白自由基形式的结构：一种新的分子模型。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3189-91. doi: 10.1073/pnas.69.11.3189.
2
Structure of oxidized flavodoxin from Anacystis nidulans.来自集胞藻6803的氧化型黄素氧还蛋白的结构
J Mol Biol. 1983 Apr 25;165(4):737-53. doi: 10.1016/s0022-2836(83)80277-0.
3
Structure of the semiquinone form of flavodoxin from Clostridum MP. Extension of 1.8 A resolution and some comparisons with the oxidized state.
J Mol Biol. 1977 Nov 25;117(1):195-225. doi: 10.1016/0022-2836(77)90031-6.
4
A comparative carbon-13, nitrogen-15, and phosphorus-31 nuclear magnetic resonance study on the flavodoxins from Clostridium MP, Megasphaera elsdenii, and Azotobacter vinelandii.
Biochemistry. 1986 Nov 4;25(22):6789-99. doi: 10.1021/bi00370a010.
5
The binding of riboflavin-5'-phosphate in a flavoprotein: flavodoxin at 2.0-Angstrom resolution.核黄素-5'-磷酸在黄素蛋白：黄素氧还蛋白中的结合，分辨率为2.0埃。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3857-60. doi: 10.1073/pnas.70.12.3857.
6
Structure and oxidation-reduction behavior of 1-deaza-FMN flavodoxins: modulation of redox potentials in flavodoxins.1-脱氮黄素单核苷酸黄素氧还蛋白的结构与氧化还原行为：黄素氧还蛋白中氧化还原电位的调节
Biochemistry. 1990 Nov 13;29(45):10364-75. doi: 10.1021/bi00497a011.
7
Dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from Clostridium MP and from Peptostreptococcus elsdenii.来自梭状芽孢杆菌MP和埃尔氏消化链球菌的黄素氧还蛋白的质子磁共振谱对氧化态的依赖性。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3292-5. doi: 10.1073/pnas.70.12.3292.
8
Resonance Raman study on the oxidized and anionic semiquinone forms of flavocytochrome b2 and L-lactate monooxygenase. Influence of the structure and environment of the isoalloxazine ring on the flavin function.黄素细胞色素b2和L-乳酸单加氧酶氧化态和阴离子半醌形式的共振拉曼研究。异咯嗪环的结构和环境对黄素功能的影响。
Biochemistry. 1997 Jul 22;36(29):8932-46. doi: 10.1021/bi962425x.
9
Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes.拜氏梭菌黄素氧还蛋白中氧化还原电位的调控：构象变化的作用
Biochemistry. 1997 Feb 11;36(6):1259-80. doi: 10.1021/bi962180o.
10
The structure of a clostridial flavodoxin, an electron-transferring flavoprotein. 3. An interpretation of an electron-density map at a nominal resolution of 3.25 Angstrom.梭菌属黄素氧还蛋白（一种电子传递黄素蛋白）的结构。3. 名义分辨率为3.25埃的电子密度图解析。
Cold Spring Harb Symp Quant Biol. 1972;36:369-80. doi: 10.1101/sqb.1972.036.01.048.

引用本文的文献

1
Crystal structure of the I-domain from the CD11a/CD18 (LFA-1, alpha L beta 2) integrin.CD11a/CD18（淋巴细胞功能相关抗原-1，αLβ2）整合素I结构域的晶体结构。
Proc Natl Acad Sci U S A. 1995 Oct 24;92(22):10277-81. doi: 10.1073/pnas.92.22.10277.
2
The binding of riboflavin-5'-phosphate in a flavoprotein: flavodoxin at 2.0-Angstrom resolution.核黄素-5'-磷酸在黄素蛋白：黄素氧还蛋白中的结合，分辨率为2.0埃。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3857-60. doi: 10.1073/pnas.70.12.3857.
3
Dependence of the proton magnetic resonance spectra on the oxidation state of flavodoxin from Clostridium MP and from Peptostreptococcus elsdenii.来自梭状芽孢杆菌MP和埃尔氏消化链球菌的黄素氧还蛋白的质子磁共振谱对氧化态的依赖性。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3292-5. doi: 10.1073/pnas.70.12.3292.
4
D-glyceraldehyde-3-phosphate dehydrogenase: three-dimensional structure and evolutionary significance.D-甘油醛-3-磷酸脱氢酶：三维结构及进化意义
Proc Natl Acad Sci U S A. 1973 Nov;70(11):3052-4. doi: 10.1073/pnas.70.11.3052.
5
Early evolution of cellular electron transport: molecular models for the ferredoxin-rubredoxin-flavodoxin region.细胞电子传递的早期演化：铁氧化还原蛋白-红素氧还蛋白-黄素氧还蛋白区域的分子模型
Orig Life. 1978 Sep;9(1):27-37. doi: 10.1007/BF00929711.
6
Handedness of crossover connections in beta sheets.β折叠中交叉连接的手性
Proc Natl Acad Sci U S A. 1976 Aug;73(8):2619-23. doi: 10.1073/pnas.73.8.2619.
7
Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.蓝色葡聚糖-琼脂糖凝胶：一种用于蛋白质中二核苷酸折叠结构的亲和柱。
Proc Natl Acad Sci U S A. 1975 Feb;72(2):669-72. doi: 10.1073/pnas.72.2.669.

本文引用的文献

1
Chemical study of two flavodoxins extracted from sulfate reducing bacteria.对从硫酸盐还原菌中提取的两种黄素氧还蛋白的化学研究。
Biochem Biophys Res Commun. 1970 Mar 12;38(5):965-72. doi: 10.1016/0006-291x(70)90816-8.
2
The structure of a clostridial flavodoxin. I. Crystallographic characterization of the oxidized and semiquinone forms.
J Biol Chem. 1969 Nov 10;244(21):6047-8.
3
Studies on flavin binding in flavodoxins.
Biochim Biophys Acta. 1971 May 12;235(2):289-302. doi: 10.1016/0005-2744(71)90207-5.
4
Molecular luminescence studies of flavins. I. The excited states of flavins.黄素的分子发光研究。I. 黄素的激发态。
J Am Chem Soc. 1972 Mar 8;94(5):1730-40. doi: 10.1021/ja00760a052.
5
Studies of flavin-protein interaction in flavoproteins using protein fluorescence and circular dichroism.
Biochemistry. 1972 Mar 14;11(6):1073-80. doi: 10.1021/bi00756a020.
6
Structure of the oxidized form of a flavodoxin at 2.5-Angstrom resolution: resolution of the phase ambiguity by anomalous scattering.2.5埃分辨率下黄素氧还蛋白氧化形式的结构：通过反常散射解决相位模糊问题。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3185-8. doi: 10.1073/pnas.69.11.3185.
7
The structure of a clostridial flavodoxin, an electron-transferring flavoprotein. 3. An interpretation of an electron-density map at a nominal resolution of 3.25 Angstrom.梭菌属黄素氧还蛋白（一种电子传递黄素蛋白）的结构。3. 名义分辨率为3.25埃的电子密度图解析。
Cold Spring Harb Symp Quant Biol. 1972;36:369-80. doi: 10.1101/sqb.1972.036.01.048.
8
The reactivity of flavoproteins with sulfite. Possible relevance to the problem of oxygen reactivity.黄素蛋白与亚硫酸盐的反应性。与氧反应性问题的可能关联。
J Biol Chem. 1969 Aug 10;244(15):3999-4006.
9
Oxidation-reduction properties of flavodoxin from Peptostreptococcus elsdenii.埃尔氏消化链球菌黄素氧还蛋白的氧化还原特性
J Biol Chem. 1969 Feb 10;244(3):803-10.

梭菌黄素氧还蛋白自由基形式的结构：一种新的分子模型。

Structure of the radical form of clostridial flavodoxin: a new molecular model.

作者信息

Andersen R D, Apgar P A, Burnett R M, Darling G D, Lequesne M E, Mayhew S G, Ludwig M L

出版信息

Proc Natl Acad Sci U S A. 1972 Nov;69(11):3189-91. doi: 10.1073/pnas.69.11.3189.

DOI:10.1073/pnas.69.11.3189
PMID:4508314
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389732/
Abstract

Interpretation of a new electron-density map at 3.25-A resolution has led to a somewhat revised model for the free radical (semiquinone) structure of flavodoxin from Clostridium MP. Although the general conformation of the molecule is very similar to that of oxidized Desulfovibrio vulgaris flavodoxin, flavin mononucleotide-protein interactions are not identical in the two flavodoxins. In the Cl. MP semiquinone molecule, the isoalloxazine ring appears to retain the essentially planar conformation characteristic of oxidized flavins; within the limits imposed by the resolution of the data, the map shows no evidence for bending of the isoalloxazine ring about N5-N10.

摘要

对分辨率为3.25埃的新电子密度图的解读，得出了来自梭菌MP的黄素氧还蛋白自由基（半醌）结构的一个略有修订的模型。尽管该分子的总体构象与氧化型普通脱硫弧菌黄素氧还蛋白非常相似，但两种黄素氧还蛋白中黄素单核苷酸与蛋白质的相互作用并不相同。在梭菌MP半醌分子中，异咯嗪环似乎保留了氧化型黄素特有的基本平面构象；在数据分辨率所限定的范围内，图谱未显示异咯嗪环围绕N5-N10弯曲的证据。