梭菌黄素氧还蛋白自由基形式的结构：一种新的分子模型。
Structure of the radical form of clostridial flavodoxin: a new molecular model.
作者信息
Andersen R D, Apgar P A, Burnett R M, Darling G D, Lequesne M E, Mayhew S G, Ludwig M L
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3189-91. doi: 10.1073/pnas.69.11.3189.
Abstract
Interpretation of a new electron-density map at 3.25-A resolution has led to a somewhat revised model for the free radical (semiquinone) structure of flavodoxin from Clostridium MP. Although the general conformation of the molecule is very similar to that of oxidized Desulfovibrio vulgaris flavodoxin, flavin mononucleotide-protein interactions are not identical in the two flavodoxins. In the Cl. MP semiquinone molecule, the isoalloxazine ring appears to retain the essentially planar conformation characteristic of oxidized flavins; within the limits imposed by the resolution of the data, the map shows no evidence for bending of the isoalloxazine ring about N5-N10.
摘要
对分辨率为3.25埃的新电子密度图的解读，得出了来自梭菌MP的黄素氧还蛋白自由基（半醌）结构的一个略有修订的模型。尽管该分子的总体构象与氧化型普通脱硫弧菌黄素氧还蛋白非常相似，但两种黄素氧还蛋白中黄素单核苷酸与蛋白质的相互作用并不相同。在梭菌MP半醌分子中，异咯嗪环似乎保留了氧化型黄素特有的基本平面构象；在数据分辨率所限定的范围内，图谱未显示异咯嗪环围绕N5-N10弯曲的证据。
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2
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J Biol Chem. 1969 Nov 10;244(21):6047-8.
3
Studies on flavin binding in flavodoxins.
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