2.5埃分辨率下黄素氧还蛋白氧化形式的结构：通过反常散射解决相位模糊问题。
Structure of the oxidized form of a flavodoxin at 2.5-Angstrom resolution: resolution of the phase ambiguity by anomalous scattering.
作者信息
Watenpaugh K D, Sieker L C, Jensen L H, Legall J, Dubourdieu M
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3185-8. doi: 10.1073/pnas.69.11.3185.
Abstract
Flavodoxin from Desulfovibrio vulgaris crystallizes in the oxidized form as well-formed, tetragonal bipyramids, space group P4(3)2(1)2, unit-cell parameters, a = b = 51.6 A, c = 139.6 A, 8 molecules per unit cell. The structure has been determined at 2.5-A resolution with phases based on a single isomorphous derivative. The phase ambiguity of a single derivative was resolved by use of anomalous scattering from the single-site Sm(+3). The molecule has a five-strand pleated sheet core with two long helices on either side of the sheet. The flavin mononucleotide lies mostly buried on one side of the molecule, but the methyl groups, one edge of the flavin, and part of the ribityl are exposed at the surface.
摘要
来自普通脱硫弧菌的黄素氧还蛋白以氧化形式结晶，形成规则的四方双锥体，空间群为P4(3)2(1)2，晶胞参数a = b = 51.6 Å，c = 139.6 Å，每个晶胞中有8个分子。该结构已在2.5 Å分辨率下基于单个同晶型衍生物确定了相位。通过利用来自单一位点Sm(+3)的反常散射解决了单个衍生物的相位模糊问题。该分子具有一个五链折叠片层核心，在片层两侧各有两条长螺旋。黄素单核苷酸大部分埋藏在分子的一侧，但甲基、黄素的一条边缘和部分核醇基暴露在表面。
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