子宫中雌二醇的动力学和平衡分析：子宫细胞中结合位点分布模型
Kinetic and equilibrium analysis of estradiol in uterus: a model of binding-site distribution in uterine cells.
作者信息
Williams D, Gorski J
出版信息
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3464-8. doi: 10.1073/pnas.69.11.3464.
Abstract
Kinetic and equilibrium binding studies indicate that the process by which the complex of estradiol-binding protein is transferred to the cell nuclei is very rapid and is readily reversible in intact cells; that is, the cytosol and nuclear binding sites are in a rapidly reversible equilibrium. Binding of the hormone appears to shift this equilibrium such that a large percent of the filled binding sites become associated with the nuclear fraction. A model is presented to show that the quantity of filled nuclear binding sites present at any estradiol concentration can be determined strictly by the initial binding between the hormone and the cytosol binding sites.
摘要
动力学和平衡结合研究表明，雌二醇结合蛋白复合物转移至细胞核的过程非常迅速，且在完整细胞中易于逆转；也就是说，胞质溶胶和细胞核结合位点处于快速可逆的平衡状态。激素的结合似乎会改变这种平衡，使得很大一部分被占据的结合位点与细胞核部分相关联。本文提出了一个模型，以表明在任何雌二醇浓度下存在的被占据的细胞核结合位点数量可严格由激素与胞质溶胶结合位点之间的初始结合来确定。
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Kinetic and equilibrium analysis of estradiol in uterus: a model of binding-site distribution in uterine cells.子宫中雌二醇的动力学和平衡分析：子宫细胞中结合位点分布模型
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本文引用的文献
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Stereospecific binding of estrogens in the rat uterus.雌激素在大鼠子宫中的立体特异性结合。
Arch Biochem Biophys. 1965 Sep;111(3):559-68. doi: 10.1016/0003-9861(65)90235-3.
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Estrogen secretion by the rat ovary in vivo during the estrous cycle and pregnancy.大鼠卵巢在发情周期和妊娠期的体内雌激素分泌。
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Subcellular distribution of 3-H-estradiol in rat uterus by quantitative autoradiography--a comparison between 3-H-estradiol and 3-H-norethynodrel.通过定量放射自显影术研究大鼠子宫中3-H-雌二醇的亚细胞分布——3-H-雌二醇与3-H-炔诺孕酮的比较
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Estrogen receptors. Quantitative studies on transfer of estradiol from cytoplasmic to nuclear binding sites.雌激素受体。关于雌二醇从细胞质结合位点向细胞核结合位点转移的定量研究。
J Biol Chem. 1971 Apr 25;246(8):2524-9.
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In vivo observations on reversible binding of estradiol-17beta,6,7-3H in rat uterus. Measurement of a "biological exchange constant" and of the "tissue capacity".大鼠子宫中雌二醇-17β,6,7-³H可逆结合的体内观察。“生物交换常数”和“组织容量”的测定。
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