Govindan M V, Spiess E, Majors J
Proc Natl Acad Sci U S A. 1982 Sep;79(17):5157-61. doi: 10.1073/pnas.79.17.5157.
Purified glucocorticoid hormone-receptor complex (HRC) from rat liver binds to specific DNA sequences contained in cloned mouse mammary tumor virus (MMTV)DNA. The binding site of the hormone-receptor complex is located in the long terminal repeat (LTR) of MMTV DNA as shown by filter binding studies with labeled restriction fragments and by visualization of DNA-receptor complexes with the electron microscope. The DNAs from cloned MMTV lacking the LTR sequences were neither retained on nitrocellulose filters nor bound specifically to HRCs examined in the electron microscope. The HRC also failed to bind to restriction fragments from pBR322 and phage lambda. Specific binding of the HRC to LTR sequences is dependent upon occupancy of the receptor by a glucocorticoid. Previous work has demonstrated that the MMTV transcription is initiated within the LTR; additionally, MMTV transcription is known to be regulated by glucocorticoids. Our present results therefore support the hypothesis that HRC regulates hormone-induced transcription by binding to specific DNA sequences near the MMTV transcription start site.
从大鼠肝脏中纯化得到的糖皮质激素激素 - 受体复合物（HRC）可与克隆的小鼠乳腺肿瘤病毒（MMTV）DNA中包含的特定DNA序列结合。通过对标记的限制性片段进行滤膜结合研究以及利用电子显微镜观察DNA - 受体复合物发现，激素 - 受体复合物的结合位点位于MMTV DNA的长末端重复序列（LTR）中。缺乏LTR序列的克隆MMTV的DNA既不能保留在硝酸纤维素滤膜上，也不能在电子显微镜下与所检测的HRC特异性结合。HRC也不能与来自pBR322和噬菌体λ的限制性片段结合。HRC与LTR序列的特异性结合依赖于糖皮质激素对受体的占据。先前的研究表明MMTV转录起始于LTR内；此外，已知MMTV转录受糖皮质激素调节。因此，我们目前的结果支持这样一种假说，即HRC通过与MMTV转录起始位点附近的特定DNA序列结合来调节激素诱导的转录。
