5-溴脱氧尿苷对汇合的成纤维细胞中染色质转录的影响。
Effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts.
作者信息
Hill B T, Tsuboi A, Baserga R
出版信息
Proc Natl Acad Sci U S A. 1974 Feb;71(2):455-9. doi: 10.1073/pnas.71.2.455.
Abstract
3T6 mouse fibroblasts were grown in 5-bromodeoxyuridine (BrdU) so that approximately 20% of the thymine residues in DNA were replaced by BrdU. BrdU replacement caused an alteration in the relative incorporation of labeled nucleotide precursors into RNA. The RNA synthesized by cells grown in BrdU has a lower proportion of adenine and a higher guanine complement. This was shown for (a) nascent RNA made in vivo by confluent monolayers of cells in culture; (b) RNA synthesized in vitro on a chromatin template with either homologous or heterologous RNA polymerase; and (c) RNA synthesized in vitro on a DNA template with a highly purified RNA polymerase. The product was completely digested by RNase. The relative decrease in the incorporation of adenine into RNA was reserved when BrdU-treated cells were allowed to proliferate in BrdU-free medium.
摘要
将3T6小鼠成纤维细胞培养在5-溴脱氧尿苷（BrdU）中，使DNA中约20%的胸腺嘧啶残基被BrdU取代。BrdU取代导致标记的核苷酸前体掺入RNA的相对量发生改变。在BrdU中培养的细胞合成的RNA中腺嘌呤的比例较低，鸟嘌呤互补物的比例较高。这在以下情况中得到了证明：（a）培养的汇合单层细胞在体内合成的新生RNA；（b）在染色质模板上用同源或异源RNA聚合酶体外合成的RNA；（c）在DNA模板上用高度纯化的RNA聚合酶体外合成的RNA。产物被核糖核酸酶完全消化。当用BrdU处理的细胞在不含BrdU的培养基中增殖时，腺嘌呤掺入RNA的相对减少得以保留。
相似文献
1
Effect of 5-bromodeoxyuridine on chromatin transcription in confluent fibroblasts.5-溴脱氧尿苷对汇合的成纤维细胞中染色质转录的影响。
Proc Natl Acad Sci U S A. 1974 Feb;71(2):455-9. doi: 10.1073/pnas.71.2.455.
2
Effect of 5-bromodeoxyuridine on the transcriptional properties of the genome in WI-38 human diploid fibroblasts.5-溴脱氧尿苷对WI-38人二倍体成纤维细胞基因组转录特性的影响。
Chem Biol Interact. 1975 May;10(5):363-75. doi: 10.1016/0009-2797(75)90058-7.
3
Accessibility of DNA in chromatin to DNA polymerase and RNA polymerase.染色质中的DNA对DNA聚合酶和RNA聚合酶的可及性。
Proc Natl Acad Sci U S A. 1973 May;70(5):1326-30. doi: 10.1073/pnas.70.5.1326.
4
Elucidation of RNA initiation (DNA promoter?) sequences in T4 DNA transcription using Escherichia coli RNA polymerase and dinucleoside monophosphates.利用大肠杆菌RNA聚合酶和二磷酸核苷对T4 DNA转录中RNA起始（DNA启动子？）序列的阐释。
Basic Life Sci. 1974;3:81-92. doi: 10.1007/978-1-4613-4529-9_7.
5
Enhancement of template activity of calf thymus and rat liver chromatin by -iirradiation.通过γ射线辐照增强小牛胸腺和大鼠肝脏染色质的模板活性。
Radiat Res. 1973 Jun;54(3):539-48.
6
Changes in the number of binding sites for ribonucleic acid polymerase in chromatin of WI-38 fibroblasts stimulated to proliferate.受刺激增殖的WI-38成纤维细胞染色质中核糖核酸聚合酶结合位点数量的变化。
Biochem J. 1974 Jul;141(1):27-34. doi: 10.1042/bj1410027.
7
RNA-dependent RNA synthesis in rat brain.大鼠脑中依赖RNA的RNA合成
Nature. 1974 May 31;249(456):445-8. doi: 10.1038/249445a0.
8
9
Differential effects of urethan on the transcriptional activity of chromatin from regenerating liver.乌拉坦对再生肝染色质转录活性的不同影响。
Cancer Res. 1974 Apr;34(4):783-7.
10
An RNA that multiplies indefinitely with DNA-dependent RNA polymerase: selection from a random copolymer.一种能借助依赖DNA的RNA聚合酶无限增殖的RNA：从随机共聚物中筛选得到。
Proc Natl Acad Sci U S A. 1973 Mar;70(3):934-8. doi: 10.1073/pnas.70.3.934.
引用本文的文献
1
Changes in cytoarchitecture and mobility in B16F1 melanoma cells induced by 5-Br-2'-dU coincide with Rock2, miRNAs 138-5p and 455-3p reciprocal expressions.5-溴-2'-脱氧尿苷诱导的B16F1黑色素瘤细胞的细胞结构和迁移性变化与Rock2、miRNAs 138-5p和455-3p的相互表达一致。
Biochem Biophys Rep. 2021 Jun 11;27:101027. doi: 10.1016/j.bbrep.2021.101027. eCollection 2021 Sep.
2
Temporal sequence of mutation for 6-thioguanine resistance in synchronised Chinese hamster cells.同步化中国仓鼠细胞中6-硫鸟嘌呤抗性突变的时间序列。
Theor Appl Genet. 1976 Jul;48(4):157-62. doi: 10.1007/BF00527366.
3
Distribution of 5-bromodeoxyuridine in the DNA of rat embryo cells.5-溴脱氧尿苷在大鼠胚胎细胞DNA中的分布。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3570-4. doi: 10.1073/pnas.71.9.3570.
4
Total substitution of bromodeoxyuridine for thymidine in the DNA of a bromodeoxyuridine-dependent cell line.在依赖溴脱氧尿苷的细胞系的DNA中，用溴脱氧尿苷完全替代胸苷。
Proc Natl Acad Sci U S A. 1974 May;71(5):2082-6. doi: 10.1073/pnas.71.5.2082.
5
Misincorporation of (TP during transcription of poly dAT-dAT and poly dABU-dABU.在聚dAT-dAT和聚dABU-dABU转录过程中（TP）的错误掺入。 （注：原文中“(TP”表述不太清晰准确，可能存在信息不完整或有误的情况）
Nucleic Acids Res. 1975 Sep;2(9):1513-23. doi: 10.1093/nar/2.9.1513.
6
Transcriptional effects of 5-bromo-2'-deoxyuridine in post-implantation mouse embryos.5-溴-2'-脱氧尿苷对植入后小鼠胚胎的转录影响。
Experientia. 1977 Apr 15;33(4):448-50. doi: 10.1007/BF01922203.
7
Hormone regulation of development in plant cells.植物细胞发育的激素调节
In Vitro. 1978 Jan;14(1):31-50. doi: 10.1007/BF02618172.
8
DNA polymerase activities in differentiating mouse neuroblastoma N-18 cells.分化中的小鼠神经母细胞瘤N - 18细胞中的DNA聚合酶活性
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1289-93. doi: 10.1073/pnas.75.3.1289.
9
Genetic aspects of bacterial endospore formation.细菌芽孢形成的遗传学方面。
Bacteriol Rev. 1976 Dec;40(4):908-62. doi: 10.1128/br.40.4.908-962.1976.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.通过乳液的液体闪烁计数法测量水溶液中的低能β发射体。
Anal Chem. 1965 Jun;37:854-7. doi: 10.1021/ac60226a017.
3
4
5-BROMODEOXYURIDINE: EFFECT ON MYOGENESIS IN VITRO.5-溴脱氧尿苷：对体外肌生成的影响。
Science. 1964 Oct 23;146(3643):533-5. doi: 10.1126/science.146.3643.533.
5
Deoxyribo ucleic acid-directed synthesis of ribonucleic acid by an enzyme from Escherichia coli.大肠杆菌中一种酶催化的脱氧核糖核酸指导的核糖核酸合成。
Proc Natl Acad Sci U S A. 1962 Jan 15;48(1):81-94. doi: 10.1073/pnas.48.1.81.
6
7
Studies in histochemistry. I. Determination of nucleic acids in microgram amounts of tissue.组织化学研究。I. 微克量组织中核酸的测定。
J Histochem Cytochem. 1956 Jan;4(1):1-10. doi: 10.1177/4.1.1.
8
A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.用于比色法测定脱氧核糖核酸的二苯胺反应的条件及机制研究。
Biochem J. 1956 Feb;62(2):315-23. doi: 10.1042/bj0620315.
9
A clonal study of the reversible inhibition of muscle differentiation by the halogenated thymidine analog 5-bromodeoxyuridine.卤代胸腺嘧啶类似物5-溴脱氧尿苷对肌肉分化可逆抑制的克隆研究。
Dev Biol. 1969 Jun;19(6):527-48. doi: 10.1016/0012-1606(69)90036-0.
10
Organ-specific restriction of transcription in mammalian chromatin.哺乳动物染色质中转录的器官特异性限制。
J Mol Biol. 1968 Jul 14;34(2):305-16. doi: 10.1016/0022-2836(68)90255-6.
Zotero 插件