Murray J, Hukins D W, Evans P
Phys Med Biol. 1979 Mar;24(2):408-15. doi: 10.1088/0031-9155/24/2/016.
The angular dependence of light scattered by spherical bacteria cannot be explained accurately by treating them as homogeneous spheres with a simple size distribution determined by electron microscopy. Calculations indicated that structural features of the bacterial cell, such as its wall, were responsible, at least in part, for the poor agreement. Thus, there is no simple theoretical model available for light scattering by bacteria. Empirical methods have, therefore, to be used to describe the curves, e.g. for antibiotic sensitivity tests. Cubic splines provide a suitable empirical method.
将球形细菌视为具有通过电子显微镜确定的简单尺寸分布的均匀球体，无法准确解释其散射光的角度依赖性。计算表明，细菌细胞的结构特征，如细胞壁，至少部分导致了这种不一致。因此，不存在适用于细菌光散射的简单理论模型。所以，必须使用经验方法来描述这些曲线，例如用于抗生素敏感性测试。三次样条提供了一种合适的经验方法。
