用于抗生素敏感性测试的光散射方法。
Light-scattering methods for antibiotic sensitivity tests.
作者信息
Murray J, Evans P, Hukins D W
出版信息
J Clin Pathol. 1980 Oct;33(10):995-1001. doi: 10.1136/jcp.33.10.995.
Abstract
The total intensity of light scattered in the angular range 30 degrees-130 degrees by a suspension of bacteria can be used as the basis of a method of antibiotic sensitivity testing. A procedure is given in which the bacteria are incubated in the presence of antibiotic, as a test, and in its absence, as a control, for 60 minutes. An incubation time of 90 minutes gives slightly more reliable results. With this increased incubation time it is necessary to measure the intensity of scattered light only at a single fixed angle or, at most, a few angles. Measuring the angular dependence of the scattered intensity does not allow the incubation period to be reduced.
摘要
细菌悬液在30度至130度角范围内散射光的总强度可作为抗生素敏感性测试方法的基础。文中给出了一种方法，将细菌在有抗生素的情况下作为试验进行培养，在无抗生素的情况下作为对照进行培养，培养60分钟。培养90分钟可得到稍更可靠的结果。随着培养时间的增加，只需在一个固定角度或最多几个角度测量散射光强度。测量散射强度的角度依赖性并不能缩短培养时间。
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Identification of bacteria by differential light scattering.通过差分光散射鉴定细菌。
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Quality control in diagnostic bacteriology.诊断细菌学中的质量控制
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