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用于抗生素敏感性测试的光散射方法。

Light-scattering methods for antibiotic sensitivity tests.

作者信息

Murray J, Evans P, Hukins D W

出版信息

J Clin Pathol. 1980 Oct;33(10):995-1001. doi: 10.1136/jcp.33.10.995.

DOI:10.1136/jcp.33.10.995
PMID:7430366
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1146302/
Abstract

The total intensity of light scattered in the angular range 30 degrees-130 degrees by a suspension of bacteria can be used as the basis of a method of antibiotic sensitivity testing. A procedure is given in which the bacteria are incubated in the presence of antibiotic, as a test, and in its absence, as a control, for 60 minutes. An incubation time of 90 minutes gives slightly more reliable results. With this increased incubation time it is necessary to measure the intensity of scattered light only at a single fixed angle or, at most, a few angles. Measuring the angular dependence of the scattered intensity does not allow the incubation period to be reduced.

摘要

细菌悬液在30度至130度角范围内散射光的总强度可作为抗生素敏感性测试方法的基础。文中给出了一种方法,将细菌在有抗生素的情况下作为试验进行培养,在无抗生素的情况下作为对照进行培养,培养60分钟。培养90分钟可得到稍更可靠的结果。随着培养时间的增加,只需在一个固定角度或最多几个角度测量散射光强度。测量散射强度的角度依赖性并不能缩短培养时间。

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1
Light-scattering methods for antibiotic sensitivity tests.用于抗生素敏感性测试的光散射方法。
J Clin Pathol. 1980 Oct;33(10):995-1001. doi: 10.1136/jcp.33.10.995.
2
Application of Mie theory and cubic splines to the representation of light scattering patterns from bacteria in the logarithmic growth phase.米氏理论和三次样条函数在对数生长期细菌光散射模式表征中的应用。
Phys Med Biol. 1979 Mar;24(2):408-15. doi: 10.1088/0031-9155/24/2/016.
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引用本文的文献

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Rapid Phenotypic Detection of Microbial Resistance in Gram-Positive Bacteria by a Real-Time Laser Scattering Method.通过实时激光散射法对革兰氏阳性菌中的微生物耐药性进行快速表型检测。
Front Microbiol. 2017 Jun 14;8:1064. doi: 10.3389/fmicb.2017.01064. eCollection 2017.
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Rapid Antimicrobial Susceptibility Testing Using Forward Laser Light Scatter Technology.使用前向激光散射技术的快速抗菌药敏试验
J Clin Microbiol. 2016 Nov;54(11):2701-2706. doi: 10.1128/JCM.01475-16. Epub 2016 Aug 24.
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Antimicrobial susceptibilities of mycobacteria as determined by differential light scattering and correlation with results from multiple reference laboratories.通过差分光散射测定分枝杆菌的抗菌药敏性及其与多个参考实验室结果的相关性。
J Clin Microbiol. 1994 Jun;32(6):1554-9. doi: 10.1128/jcm.32.6.1554-1559.1994.

本文引用的文献

1
Turbidimetric assays: the antibiotic dose-response line.比浊法测定:抗生素剂量反应线
Appl Microbiol. 1968 May;16(5):777-80. doi: 10.1128/am.16.5.777-780.1968.
2
Rapid detection of penicillin sensitivity in Staphylococcus aureus.金黄色葡萄球菌中青霉素敏感性的快速检测
Nature. 1970 Oct 31;228(5270):458-60. doi: 10.1038/228458a0.
3
Identification of bacteria by differential light scattering.通过差分光散射鉴定细菌。
Nature. 1969 Mar 29;221(5187):1257-8. doi: 10.1038/2211257a0.
4
Quality control in diagnostic bacteriology.诊断细菌学中的质量控制
Proc R Soc Med. 1968 May;61(5):457-63. doi: 10.1177/003591576806100521.
5
Clinical laboratory use of differential light scattering. I. Antibiotic susceptibility testing.临床实验室中差分光散射的应用。I. 抗生素敏感性测试。
Clin Chem. 1973 Aug;19(8):883-90.
6
Laser light scattering bioassay for veterinary drug residues in food producing animals. 1. Dose-response results for milk, serum, urine, and bile.用于食品生产动物兽药残留检测的激光光散射生物测定法。1. 牛奶、血清、尿液和胆汁的剂量反应结果。
J Agric Food Chem. 1976 Sep-Oct;24(5):984-8. doi: 10.1021/jf60207a002.
7
Application of Mie theory and cubic splines to the representation of light scattering patterns from bacteria in the logarithmic growth phase.米氏理论和三次样条函数在对数生长期细菌光散射模式表征中的应用。
Phys Med Biol. 1979 Mar;24(2):408-15. doi: 10.1088/0031-9155/24/2/016.