Bianchi G, Celentano F, Cortili G, Orsenigo M N, Torelli G
J Biochem Biophys Methods. 1979 May;1(2):91-104. doi: 10.1016/0165-022x(79)90016-2.
A method allowing the measurement of the electrical quantities related to the physiological functions of the frog skin in situ is presented. The method allows the performance of several experiments on the same pithed animal, which remains alive for a number of days. The preparation is very stable, and the electrical potential difference and short-circuit current values are higher than in isolated skin. The theory of measurement and the possible systematic errors are discussed. The possibilities of the method are evaluated on comparing the pH and temperature dependence of the electrical quantities in situ with previous measurements on isolated skin.
本文介绍了一种能够测量与青蛙皮肤原位生理功能相关电参数的方法。该方法允许在同一去脑动物身上进行多项实验，该动物可存活数天。制备物非常稳定，其电位差和短路电流值高于离体皮肤。文中讨论了测量原理和可能存在的系统误差。通过比较原位电参数的pH值和温度依赖性与先前对离体皮肤的测量结果，对该方法的可能性进行了评估。
