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两种提取链球菌T抗原方法的研究。

Studies on two methods for extraction of streptococcal T antigens.

作者信息

Erwa H H

出版信息

J Hyg (Lond). 1973 Mar;71(1):131-8. doi: 10.1017/s0022172400046295.

DOI:10.1017/s0022172400046295
PMID:4511945
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2130443/
Abstract

The extraction of group A streptococcal antigens by group C phage-associated lysin has been confirmed. In addition to the T antigen the extract contained M-protein, group-specific polysaccharide and mucopeptide antigen which was difficult to remove. This method of extraction of the T antigen was compared with the trypsin method. The latter method was found to be of advantage in giving a pure specific antigen.

摘要

已证实用C组噬菌体相关溶素提取A组链球菌抗原。提取物中除T抗原外，还含有M蛋白、群特异性多糖和难以去除的粘肽抗原。将这种提取T抗原的方法与胰蛋白酶法进行了比较。发现后一种方法在获得纯特异性抗原方面具有优势。

相似文献

1
Studies on two methods for extraction of streptococcal T antigens.两种提取链球菌T抗原方法的研究。
J Hyg (Lond). 1973 Mar;71(1):131-8. doi: 10.1017/s0022172400046295.
2
Studies on the bacteriophages of hemolytic streptococci. II. Antigens released from the streptococcal cell wall by a phage-associated lysin.溶血性链球菌噬菌体的研究。II. 由噬菌体相关溶素从链球菌细胞壁释放的抗原。
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3
Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.噬菌体相关溶素从A组1型链球菌细胞壁释放的M蛋白的制备及其抗原性
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Immunogenicity and characteristics of M protein released by phage-associated lysin from group-A streptococci types 1 and 23.1型和23型A组链球菌噬菌体相关溶素释放的M蛋白的免疫原性及特性
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Loss of macrophages from peritoneal exudates of sensitized rats after injection of streptococcal antigens.
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引用本文的文献

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2
Simple procedure for production by group C streptococci of phage-associated lysin active against group A streptococci.C组链球菌产生对A组链球菌具有活性的噬菌体相关溶素的简单方法。
Appl Microbiol. 1975 Feb;29(2):175-8. doi: 10.1128/am.29.2.175-178.1975.
3
T antigen of Streptococcus pyogenes: isolation and purification.化脓性链球菌的T抗原：分离与纯化。
Infect Immun. 1976 Mar;13(3):993-4. doi: 10.1128/iai.13.3.993-994.1976.
4
Characterization of group A streptococcal T-12 protein purified by ion-exchange column chromatography.通过离子交换柱色谱法纯化的A组链球菌T-12蛋白的特性分析。
Infect Immun. 1978 Sep;21(3):940-5. doi: 10.1128/iai.21.3.940-945.1978.
5
Purification and characterization of group A streptococcal T-1 antigen.A 组链球菌 T-1 抗原的纯化与特性分析
Infect Immun. 1977 Jun;16(3):867-75. doi: 10.1128/iai.16.3.867-875.1977.

本文引用的文献

1
Extraction of the T antigen of Streptococcus pyogenes.化脓性链球菌T抗原的提取。
J Gen Microbiol. 1951 Oct;5(4):640-7. doi: 10.1099/00221287-5-4-640.
2
Studies of the bacterial cell wall. II. Methods of preparation and some properties of cell walls.细菌细胞壁的研究。II. 细胞壁的制备方法及一些性质
Biochim Biophys Acta. 1951 Jul;7(2):177-97. doi: 10.1016/0006-3002(51)90017-0.
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IMMUNOLOGICAL PROPERTIES OF BACTERIAL CELL WALL MUCOPEPTIDES.细菌细胞壁粘肽的免疫学特性
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4
Studies on the chemical structure of the streptococcal cell wall. I. The identification of a mucopeptide in the cell walls of groups A and A-variant streptococci.链球菌细胞壁化学结构的研究。I. A组和A变异组链球菌细胞壁中一种粘肽的鉴定。
J Exp Med. 1961 Jul 1;114(1):127-40. doi: 10.1084/jem.114.1.127.
5
Preparation and antigenicity of M protein released from group A, type 1 streptococcal cell walls by phage-associated lysin.噬菌体相关溶素从A组1型链球菌细胞壁释放的M蛋白的制备及其抗原性
J Exp Med. 1960 Jul 1;112(1):77-96. doi: 10.1084/jem.112.1.77.
6
Studies on bacteriophages of hemolytic streptococci. I. Factors influencing the interaction of phage and susceptible host cell.溶血性链球菌噬菌体的研究。I. 影响噬菌体与易感宿主细胞相互作用的因素。
J Exp Med. 1957 Sep 1;106(3):365-84. doi: 10.1084/jem.106.3.365.
7
The active agent in nascent phage lysis of streptococci.链球菌新生噬菌体裂解中的活性因子。
J Gen Microbiol. 1957 Jun;16(3):584-95. doi: 10.1099/00221287-16-3-584.
8
Identification of strains of Streptococcus pyogenes of Types 5, 11, 12, 27 and 44 by the precipitin test for the T antigen.通过T抗原沉淀试验鉴定5型、11型、12型、27型和44型化脓性链球菌菌株。
J Gen Microbiol. 1953 Aug;9(1):110-8. doi: 10.1099/00221287-9-1-110.
9
A latex agglutination test for the measurement of antibodies to group-specific streptococcal polysaccharides.一种用于检测抗A组特异性链球菌多糖抗体的乳胶凝集试验。
Clin Exp Immunol. 1969 Mar;4(3):311-21.
10
An immunochemical analysis of bacterial mucopeptides.细菌粘肽的免疫化学分析。
J Immunol. 1966 Dec;97(6):797-804.