Cohen J O, Gross H, Harrell W K
Appl Microbiol. 1975 Feb;29(2):175-8. doi: 10.1128/am.29.2.175-178.1975.
Phage-associated lysin of high potency was prepared by growing the host group C streptococcal strain 26RP66 in a semisynthetic medium. The lysin was stabilized by adding dithiothreitol and neutralized ethylenediaminetetraacetic acid (EDTA) to facilitate further concentration and partial purification. The lysin remained active when stored at -65 C for 1 year. Lysin was active against all strains of group A streptococci tested and was more active against living cells than heat-killed cells. The procedure outlined is practicable for most bacteriological research laboratories and does not require column purification or other complex biochemical procedures. It should be useful to any laboratory which requires small amounts of lysin to produce L-forms and protoplasts or to release streptococcal antigens.
通过在半合成培养基中培养宿主C组链球菌菌株26RP66制备高效噬菌体相关溶素。通过添加二硫苏糖醇和中和的乙二胺四乙酸（EDTA）来稳定溶素，以利于进一步浓缩和部分纯化。该溶素在-65℃储存1年仍保持活性。溶素对所有测试的A组链球菌菌株均有活性，且对活细胞的活性比对热杀死细胞的活性更高。所述方法对大多数细菌学研究实验室而言是可行的，并且不需要柱纯化或其他复杂的生化程序。对于任何需要少量溶素来产生L型菌和原生质体或释放链球菌抗原的实验室都应该是有用的。
