Castro A, Cid A, Buschbaum P A, Clark L
Res Commun Chem Pathol Pharmacol. 1979 Jun;24(3):583-93.
We present a new method for the determination of serum vitamin B12 concentration by competitive protein binding using 57Co labeled vitamin B12, chicken serum (CS) as the binding protein, and a sephadex column separation. We placed a mixture of extracted sample, labeled B12, and CS binder above an A-25 sephadex column, and after 1 hour incubation at room temperature drained the column and washed it twice with buffer. We counted the free fraction remaining in each column. Within run C.V.'s for low and high values in the normal range were 10.6% and 6.03%. The between run C.V.'s were 7.7% and 7.5% respectively. The values obtained for 95 clinical specimens correlated well with those for a reference method.
我们介绍一种通过竞争性蛋白结合法测定血清维生素B12浓度的新方法，该方法使用57Co标记的维生素B12、鸡血清（CS）作为结合蛋白，并采用葡聚糖凝胶柱分离。我们将提取的样品、标记的B12和CS结合剂的混合物置于A - 25葡聚糖凝胶柱上方，在室温下孵育1小时后排出柱内液体，并用缓冲液洗涤两次。我们对每根柱中剩余的游离部分进行计数。在正常范围内，低浓度和高浓度值的批内变异系数分别为10.6%和6.03%。批间变异系数分别为7.7%和7.5%。95份临床标本的测定值与参考方法的测定值相关性良好。
