Kubiatowicz D O, Ithakissios D S, Windorski D C
Clin Chem. 1977 Jun;23(6):1037-45.
We describe a vitamin B12 radioassay in which oyster toadfish (Opsanus tau) serum is used as the binding protein. The serum is quite stable and has a high capacity and high binding affinity (K greater than 10(12) liter/mol) for vitamin B12. The binding is not significantly affected by temperature, the presence of denatured proteins, or the amount of vitamin B12 present. The radioassay is reproducible (CV 4.4%) within assay, 7.8% between assays) and sensitive (12 ng/liter). Assay accuracy is unaffected by the protein in serum or by reasonable variations in incubation temperature and time.
我们描述了一种维生素B12放射免疫测定法，其中使用牡蛎蟾鱼（Opsanus tau）血清作为结合蛋白。该血清相当稳定，对维生素B12具有高容量和高结合亲和力（K大于10¹²升/摩尔）。结合不受温度、变性蛋白质的存在或维生素B12含量的显著影响。该放射免疫测定法在测定内具有可重复性（CV为4.4%），不同测定之间为7.8%）且灵敏度高（12纳克/升）。测定准确性不受血清中蛋白质的影响，也不受孵育温度和时间的合理变化的影响。
