Schölmerich J, Kremer B, Richter I E, Schmidt K, Setyadharma H, Schoenenberger G A
Scand J Plast Reconstr Surg. 1979;13(1):223-30. doi: 10.3109/02844317909013061.
Studies on isolated perfused rat livers 5 days after either a sublethal burn or an i.p. injection of human/mouse burn toxin showed a significant inhibition of the glucose/urea synthesis and the ATP production concomitantly with ultrastructural mitochondrial damages. A direct specific effect of these burn toxins on enzymatically isolated liver parenchyma cells was found either after direct incubation of the isolated cells with the compound or 5 days after injection of the toxin to the animals followed by the isolation of the cells. Control experiments were performed with the "native" non-toxic precursor from normal skin. Liver cells of rats pretreated with the toxin showed an 100% increase of the amino-acid release while this increase was 70% after direct toxin incubation. Glycogen synthesis from lactate, alanin and fructose was significantly decreased in both toxin groups while the glucose synthesis was not altered. The degree of the inhibition of the glycogen synthesis was directly correlated to the number of ATP-dependent metabolic steps. A disturbance of the oxygen transfer system by structural damages of the mitochondria seems to be the basic mechanism for these specific metabolic alterations due to ultrastructural mitochondrial damages.
对亚致死性烧伤或腹腔注射人/鼠烧伤毒素5天后的离体灌注大鼠肝脏进行的研究表明，葡萄糖/尿素合成及ATP生成受到显著抑制，同时伴有线粒体超微结构损伤。在将分离的细胞与该化合物直接孵育后，或者在给动物注射毒素5天后分离细胞时，发现这些烧伤毒素对酶法分离的肝实质细胞有直接特异性作用。用来自正常皮肤的“天然”无毒前体进行了对照实验。用毒素预处理的大鼠肝细胞的氨基酸释放增加了100%，而直接进行毒素孵育后这一增加为70%。在两个毒素组中，由乳酸、丙氨酸和果糖合成糖原均显著减少，而葡萄糖合成未改变。糖原合成的抑制程度与ATP依赖的代谢步骤数量直接相关。线粒体结构损伤导致的氧转运系统紊乱似乎是这些因线粒体超微结构损伤引起的特异性代谢改变的基本机制。
