Huggins C B, Reddi A H
Proc Natl Acad Sci U S A. 1973 Mar;70(3):929-33. doi: 10.1073/pnas.70.3.929.
Optimal amounts of demineralized bone matrix possess the ability to coagulate platelet-free heparinized, citrated, and oxalated blood plasmas of guinea pigs. Clotting constituents become denatured in contact with the insoluble coagulant proteins. Quantities in excess of optimal modify plasma so that it does not gel when thrombin is added. The newly described coagulant effects are not restricted to the bone matrix, but are present also in the demineralized matrices of tooth and ivory, and in denatured tendon as well. They are regulated properties that were not demonstrated in mineralized bone or native tendon. The coagulant attributes of bone matrix are consistent with those of electropositive polymers of a specific sort.
适量的脱矿骨基质能够使豚鼠的无血小板肝素化、枸橼酸化和草酸盐化血浆发生凝固。凝血成分在与不溶性凝血蛋白接触时会发生变性。超过最佳量的脱矿骨基质会改变血浆，以至于加入凝血酶后血浆不会凝胶化。新描述的凝血作用并不局限于骨基质，在牙齿和象牙的脱矿基质以及变性肌腱中也存在。它们是矿化骨或天然肌腱中未表现出的可调节特性。骨基质的凝血特性与特定类型的正电聚合物的特性一致。
