Kessels H, Béguin S, Andree H, Hemker H C
Cardiovascular Research Institute Maastricht, Dept. of Biochemistry, University of Limburg, The Netherlands.
Thromb Haemost. 1994 Jul;72(1):78-83.
A technique has been developed to monitor the development of thrombin in freshly collected whole blood in the absence of anticoagulants. It is based on the centrifugal separation of the cellular components from subsamples of blood drawn from non-anticoagulated clotting whole blood which are diluted in buffer containing a chromogenic substrate. It is shown that the burst of thrombin generation after triggering coagulation with trace amounts of tissue thromboplastin occurs sooner in non-anticoagulated whole blood than in citrated whole blood. Heparin is shown to prolong the lag-time of thrombin generation more in native blood than in recalcified citrated blood. It is also demonstrated that intake of 500 mg of aspirin significantly delays and inhibits thrombin generation in non-anticoagulated, thromboplastin triggered whole blood, whereas it has no effect on the coagulation in citrated plasma. The effect of aspirin intake on thrombin generation in blood is roughly equal to that of 0.03 U/ml of unfractionated heparin. This demonstrates that platelet reactions and the coagulation system are closely linked processes. It further lends support to the hypothesis that inhibition of thrombin generation is a common denominator of antithrombotic therapy.
已开发出一种技术，可在不使用抗凝剂的情况下监测新鲜采集的全血中凝血酶的生成情况。该技术基于从未抗凝的凝血全血抽取的血样中进行细胞成分的离心分离，这些血样在含有发色底物的缓冲液中稀释。结果表明，用微量组织凝血活酶触发凝血后，未抗凝全血中凝血酶生成的爆发比枸橼酸化全血中出现得更早。肝素在天然血液中比在重新钙化的枸橼酸血液中更能延长凝血酶生成的延迟时间。还证明，摄入500毫克阿司匹林会显著延迟并抑制未抗凝、凝血活酶触发的全血中凝血酶的生成，而对枸橼酸血浆中的凝血没有影响。摄入阿司匹林对血液中凝血酶生成的影响大致相当于0.03 U/ml普通肝素的影响。这表明血小板反应和凝血系统是紧密相连的过程。这进一步支持了以下假设：抑制凝血酶生成是抗血栓治疗的一个共同特征。
