Reddi A H, Huggins C B
Proc Natl Acad Sci U S A. 1974 May;71(5):1648-52. doi: 10.1073/pnas.71.5.1648.
Brief exposure of rat bone matrix to highly charged electrochemicals had profound but reversible effects on its competence to transform fibroblasts into chondroblasts and osteoblasts. Suppression and subsequent reactivation of this function were influenced critically by the charge of the electrochemical reagent and the pH of the reaction mixture. In dilute acids, Evans Blue, a long electronegative molecule, suppressed transforming competence, whereas hexadimethrine, a polycationic quaternary ammonium base, failed to do so. A cycle of inactivation-restoration of transforming competence was achieved by sequential treatment of bone matrix with Evans Blue followed by hexadimethrine; an electrochemical complex was demonstrated by histochemistry. In dilute alkalies, hexadimethrine inhibited, whereas, Evans Blue did not suppress the transforming function of the bone matrix. Electric charge characteristics on the surface of the bone matrix are of crucial significance for the fibroblast-transforming attribute.
将大鼠骨基质短暂暴露于高电荷电化学物质中，会对其将成纤维细胞转化为软骨细胞和成骨细胞的能力产生深刻但可逆的影响。这种功能的抑制和随后的重新激活受到电化学试剂的电荷以及反应混合物pH值的关键影响。在稀酸中，伊文思蓝（一种长链带负电的分子）会抑制转化能力，而聚阳离子季铵碱六甲双铵则不会。通过先用伊文思蓝处理骨基质，然后用六甲双铵处理，实现了转化能力失活-恢复的循环；通过组织化学证明了一种电化学复合物的存在。在稀碱中，六甲双铵会抑制，而伊文思蓝不会抑制骨基质的转化功能。骨基质表面的电荷特性对于成纤维细胞转化特性至关重要。
