Machida Y, Bloch K
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1146-8. doi: 10.1073/pnas.70.4.1146.
The mycobacterial polysaccharides MMP and MGLP, which contain numerous O-methyl-sugar residues, markedly stimulate fatty acid synthesis catalyzed by a multienzyme complex from Mycobacterium phlei [Ilton, M. et al. (1971) Proc. Nat. Acad. Sci. USA 68, 87-91]. When aqueous solutions containing MMP or MGLP and palmitoyl-CoA were chromatographed on Sephadex G-75 under conditions that widely separate the individual components, polysaccharide and fatty acyl-CoA were eluted in a single peak, indicating formation of a molecular complex. Similarly, the mycobacterial polysaccharides associate with the CoA derivatives of C(18), C(20), and C(22) acids to yield complexes containing maximally 1 mol of fatty acyl-CoA per mol of polysaccharide. The formation of these novel complexes may result from hydrophobic interactions between the paraffin chains of the acyl-CoA derivatives and O-methyl-sugar residues of the polysaccharides.
分枝杆菌多糖MMP和MGLP含有大量O-甲基糖残基，能显著刺激由草分枝杆菌的多酶复合物催化的脂肪酸合成[伊尔通，M.等人（1971年）《美国国家科学院院刊》68，87 - 91]。当含有MMP或MGLP以及棕榈酰辅酶A的水溶液在能使各成分充分分离的条件下在葡聚糖G - 75上进行色谱分析时，多糖和脂肪酰辅酶A在单一峰中被洗脱，表明形成了分子复合物。同样，分枝杆菌多糖与C(18)、C(20)和C(22)酸的辅酶A衍生物结合，形成每摩尔多糖最多含1摩尔脂肪酰辅酶A的复合物。这些新型复合物的形成可能是由于酰基辅酶A衍生物的石蜡链与多糖的O - 甲基糖残基之间的疏水相互作用。
