一种分枝杆菌多糖-脂肪酰辅酶A复合物的结构：核磁共振研究
Structure of a mycobacterial polysaccharide-fatty acyl-CoA complex: nuclear magnetic resonance studies.
作者信息
Maggio J E
出版信息
Proc Natl Acad Sci U S A. 1980 May;77(5):2582-6. doi: 10.1073/pnas.77.5.2582.
Abstract
MMP, a linear alpha 1 leads to 4 linked polymer of 3-O-methylmannose, regulates the fatty acid synthetase from Mycobacterium smegmatis by forming stoichiometric complexes with the long-chain acyl-CoA synthetase products. In agreement with previous proposals [Bloch, K. (1977) in Advances in Enzymology and Related Areas of Molecular Biology, ed. Meister, A. (Wiley, New York), Vol. 45, pp. 1-84], nuclear magnetic resonance studies show that the polysaccharide, a random coil in its free form, undergoes a major conformational transition upon enclosing long-chain acyl-CoA. The polysaccharide, probably in helical conformation in the complexed form, interacts with both the paraffinic chain and the CoA moieties of the included fatty acyl thioester.
摘要
MMP是一种由3 - O - 甲基甘露糖构成的线性α1至4连接的聚合物，它通过与长链酰基辅酶A合成酶产物形成化学计量复合物来调节耻垢分枝杆菌的脂肪酸合成酶。与之前的提议一致[布洛赫，K.（1977年），载于《酶学及分子生物学相关领域进展》，编者迈斯特，A.（威利出版社，纽约），第45卷，第1 - 84页]，核磁共振研究表明，多糖在其自由形式下是无规卷曲，在包裹长链酰基辅酶A时会发生主要的构象转变。该多糖在复合形式下可能呈螺旋构象，与所含脂肪酰硫酯的石蜡链和辅酶A部分相互作用。
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