直接测量人类血红蛋白中碱性玻尔基团的pK值。
Direct measurement of the pK values of an alkaline Bohr group in human hemoglobin.
作者信息
Kilmartin J V, Breen J J, Roberts G C, Ho C
出版信息
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1246-9. doi: 10.1073/pnas.70.4.1246.
Abstract
Proton nuclear magnetic resonance spectra of normal and des-(his 146beta) human hemoglobin in the aromatic resonance region have been compared at different pH values. From these measurements, a pK value for histidine 146beta of 7.1 in carbonmonoxyhemoglobin and 8.0 in deoxyhemoglobin has been found. These pK values confirm the proposed role of histidine 146beta in the alkaline Bohr effect.
摘要
已在不同pH值下比较了正常血红蛋白和去（组氨酸146β）人血红蛋白在芳香族共振区域的质子核磁共振谱。通过这些测量，发现碳氧血红蛋白中组氨酸146β的pK值为7.1，脱氧血红蛋白中为8.0。这些pK值证实了组氨酸146β在碱性玻尔效应中所提出的作用。
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