一些对脱氧核糖核酸具有高亲和力的染色质酸性蛋白的结合参数。
Some binding parameters of chromatin acidic proteins with high affinity for deoxyribonucleic acid.
作者信息
Patel G L, Thomas T L
出版信息
Proc Natl Acad Sci U S A. 1973 Sep;70(9):2524-8. doi: 10.1073/pnas.70.9.2524.
Abstract
A fraction of chromatin acidic protein from rat liver, predominantly comprised of low-molecular-weight species, showed highest in vitro affinity for DNA when compared with other fractions of nuclear acidic protein. Maximum binding of this protein fraction was observed at physiological ionic strength of 0.14 M NaCl and DNA/protein ratio > 1. Comparison of their binding to homologous and heterologous DNAs showed partial species specificity. All molecular species of this protein fraction appear to bind to DNA.
摘要
大鼠肝脏染色质酸性蛋白的一部分，主要由低分子量物质组成，与其他核酸性蛋白组分相比，在体外对DNA表现出最高的亲和力。在0.14M NaCl的生理离子强度和DNA/蛋白质比率>1时观察到该蛋白质组分的最大结合。它们与同源和异源DNA结合的比较显示出部分物种特异性。该蛋白质组分的所有分子物种似乎都与DNA结合。
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