Preisler H S, Housman D, Scher W, Friend C
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2956-9. doi: 10.1073/pnas.70.10.2956.
Friend leukemia cells grown in the presence of dimethyl sulfoxide show enhanced erythroid differentiation and hemoglobin synthesis. The effects of dimethyl sulfoxide stimulation are inhibited by BrdU. To determine the effect of BrdU on the amount of globin mRNA present in cells treated and not treated with dimethyl sulfoxide, molecular hybridization between total cell RNA and [(3)H]DNA complementary to mouse globin mRNA was used. Cells treated with BrdU and dimethyl sulfoxide had 70% less globin mRNA than cells treated with dimethyl sulfoxide alone. The size and base sequence of the residual globin mRNA in the cultures treated with BrdU and dimethyl sulfoxide were unaltered. Cells treated with BrdU alone contained slightly more globin mRNA than did the untreated controls, suggesting that BrdU may have a dual effect in transcription of messenger RNA.
在二甲基亚砜存在的情况下培养的Friend白血病细胞表现出增强的红系分化和血红蛋白合成。二甲基亚砜刺激的作用被溴脱氧尿苷（BrdU）抑制。为了确定BrdU对用二甲基亚砜处理和未处理的细胞中珠蛋白mRNA含量的影响，使用了总细胞RNA与与小鼠珠蛋白mRNA互补的[(3)H]DNA之间的分子杂交。用BrdU和二甲基亚砜处理的细胞比仅用二甲基亚砜处理的细胞珠蛋白mRNA少70%。用BrdU和二甲基亚砜处理的培养物中残留珠蛋白mRNA的大小和碱基序列未改变。单独用BrdU处理的细胞比未处理的对照含有略多的珠蛋白mRNA，这表明BrdU在信使RNA转录中可能具有双重作用。
