珠蛋白信使核糖核酸形成的原位定位。II. 用二甲基亚砜处理弗氏病毒转化的小鼠细胞后。
In situ localization of globin messenger RNA formation. II. After treatment of Friend virus-transformed mouse cells with dimethyl sulfoxide.
作者信息
Conkie D, Affara N, Harrison P R, Paul J, Jones K
出版信息
J Cell Biol. 1974 Nov;63(2 Pt 1):414-9. doi: 10.1083/jcb.63.2.414.
Abstract
Globin messenger RNA (mRNA) levels in Friend virus-transformed mouse cells have been estimated by in situ hybridization of DNA copy (cDNA) to fixed preparations of cells and by hybridization of cDNA to extracted cytoplasmic RNA in true solution. The results obtained by both methods agree in showing that a low level of globin mRNA can be detected in untreated Friend cells. The levels of hemoglobin and globin mRNA have also been correlated after treatment of Friend cells with dimethyl sulfoxide (DMSO). The results obtained by both experimental approaches show that there is a minimum period of treatment with DMSO required in order that Friend cells may become hemoglobinized, and that this period coincides with the time when globin mRNA accumulates. Moreover, bromodeoxyuridine prevents both hemoglobin and globin mRNA accumulation.
摘要
通过将DNA拷贝（cDNA）与细胞固定制剂进行原位杂交以及将cDNA与真实溶液中提取的细胞质RNA进行杂交，对弗氏病毒转化的小鼠细胞中的珠蛋白信使核糖核酸（mRNA）水平进行了估算。两种方法得到的结果一致表明，在未处理的弗氏细胞中可检测到低水平的珠蛋白mRNA。在用二甲基亚砜（DMSO）处理弗氏细胞后，血红蛋白和珠蛋白mRNA的水平也具有相关性。两种实验方法得到的结果均表明，DMSO处理需要一段最短时间，弗氏细胞才能实现血红蛋白化，且这段时间与珠蛋白mRNA积累的时间相吻合。此外，溴脱氧尿苷可阻止血红蛋白和珠蛋白mRNA的积累。
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本文引用的文献
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