Schumacher H R, Szekely I E, Park S A, Rao U N, Fisher D R, Patel S B
Am J Pathol. 1973 Oct;73(1):27-46.
Quantitative and qualitative electron microscopic studies were performed on the leukemic cells of 3 patients with stem cell leukemia, 6 patients with acute lymphoblastic leukemia, 7 patients with acute monoblastic leukemia, 3 patients with acute myeloblastic leukemia and 7 patients with acute monomyeloblastic leukemia. Significant quantitative differences were noted between some of the leukemic cells in heterochromatin:euchromatin ratios, cell size, granules per cell, amount of endoplasmic reticulum and the number of polyribosomes. Qualitative abnormalities which were found in some cells of all the leukemic groups were observed. These abnormalities included nuclear pockets, deep nuclear indentations (usually not the stem cell), nucleosomes, dilated perinuclear spaces, centrioles located in nuclear pockets (not in the stem cell or lymphoblast), accumulation of microfibrils (greatest in the monoblast and myeloblast), disrupted mitochondria with virus-like particles (none in stem cells), smaller granules and mitochondrial DNA, glycogen, and myelin whorls. The presence of the smaller granules in disrupted mitochondria and the resulting clear areas is probably related to the deranged carbohydrate metabolism of these cells. The presence of virus-like particles within mitochondria may be extremely important, but requires much more investigation. Some of the above findings may offer clues to further investigation of the acute leukemic cell.
对3例干细胞白血病、6例急性淋巴细胞白血病、7例急性单核细胞白血病、3例急性粒细胞白血病和7例急性粒单核细胞白血病患者的白血病细胞进行了定量和定性电子显微镜研究。在一些白血病细胞的异染色质与常染色质比例、细胞大小、每个细胞的颗粒、内质网数量和多聚核糖体数量方面发现了显著的定量差异。在所有白血病组的一些细胞中都发现了定性异常。这些异常包括核袋、核深切迹（通常不是干细胞）、核小体、核周间隙增宽、位于核袋中的中心粒（不在干细胞或淋巴母细胞中）、微原纤维聚集（在单核母细胞和成髓细胞中最明显）、带有病毒样颗粒的线粒体破坏（干细胞中没有）、较小的颗粒以及线粒体DNA、糖原和髓鞘样小体。线粒体破坏时出现较小颗粒以及由此产生的透明区域可能与这些细胞紊乱的碳水化合物代谢有关。线粒体中存在病毒样颗粒可能极其重要，但需要更多的研究。上述一些发现可能为进一步研究急性白血病细胞提供线索。
