哺乳动物细胞DNA在碱性蔗糖梯度中的分子特性
Molecular nature of mammalian cell DNA in alkaline sucrose gradients.
作者信息
Simpson J R, Nagle W A, Bick M D, Belli J A
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3660-4. doi: 10.1073/pnas.70.12.3660.
Abstract
Mammalian cell DNA that exhibited anomalous sedimentation in alkaline sucrose gradients was examined directly by electron microscopy. Its appearance was that of duplex DNA. In addition, some duplex DNA was observed under conditions in which the sedimentation anomaly was no longer apparent. Persistence of double-stranded DNA under denaturing conditions suggests caution in the interpretation of changes in the molecular weight or conformation of DNA based solely on analysis of sedimentation profiles.
摘要
通过电子显微镜直接检查了在碱性蔗糖梯度中呈现异常沉降的哺乳动物细胞DNA。其外观为双链DNA。此外，在沉降异常不再明显的条件下也观察到了一些双链DNA。在变性条件下双链DNA的持续存在表明，仅基于沉降图谱分析来解释DNA分子量或构象的变化时需谨慎。
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