BHK - 21 - C13细胞中脱氧核糖核酸的合成
Synthesis of deoxyribonucleic acid in BHK-21-C13 cells.
作者信息
Hayton G J, Pearson C K, Scaife J R, Keir H M
出版信息
Biochem J. 1973 Mar;131(3):499-508. doi: 10.1042/bj1310499.
Abstract
Newly synthesized (3)H-labelled DNA was extracted from baby hamster kidney cells (BHK-21/C13 cells) and was shown to possess single-stranded properties when examined by column chromatography on benzoylated naphthoylated DEAE-cellulose, hydroxyapatite and methylated albumin on kieselguhr, and by its affinity for nitrocellulose filters. Some of the newly synthesized DNA was shown to be of lower molecular weight than the bulk of the DNA when examined by alkaline sucrose-density-gradient centrifugation. The properties observed were not affected by treatment of the DNA with ribonuclease, Pronase or amylase. The effect of the size of the DNA on its observed properties was examined and is discussed. It is concluded that DNA synthesis in BHK-21/C13 cells proceeds according to the discontinuous-mechanism model in at least one of the strands.
摘要
从幼仓鼠肾细胞（BHK - 21/C13细胞）中提取新合成的（3）H标记DNA，通过在苯甲酰化萘甲酰化二乙氨基乙基纤维素、羟基磷灰石和硅藻土上的甲基化白蛋白柱色谱分析，以及其对硝酸纤维素滤膜的亲和力检测，结果表明其具有单链性质。通过碱性蔗糖密度梯度离心法检测发现，一些新合成的DNA分子量低于大部分DNA。用核糖核酸酶、链霉蛋白酶或淀粉酶处理DNA后，所观察到的性质未受影响。研究并讨论了DNA大小对其观察到的性质的影响。得出的结论是，BHK - 21/C13细胞中的DNA合成至少在一条链上按照不连续机制模型进行。
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