从人宫颈癌细胞系（HeLa）线粒体DNA转录的含离散聚腺苷酸RNA成分的鉴定。
Identification of discrete polyadenylate-containing RNA components transcribed from HeLa cell mitochondrial DNA.
作者信息
Ojala D, Attardi G
出版信息
Proc Natl Acad Sci U S A. 1974 Feb;71(2):563-7. doi: 10.1073/pnas.71.2.563.
Abstract
Polyacrylamide gel electrophoresis and sedimentation analysis under denaturing conditions of poly(A)-containing RNA from the polysome region of the sedimentation pattern of a HeLa-cell mitochondrial lysate has revealed the occurrence of a discrete RNA component, which sediments in the native state with a sedimentation constant of about 7 S. From the sedimentation behavior under native and denaturing conditions and the poly(A) content, a molecular weight of about 9 x 10(4) has been estimated for this component. RNA.DNA hybridization experiments have indicated that this component is coded for by the light strand of mitochondrial DNA. Evidence for the occurrence of a poly(A)-containing RNA component sedimenting at about 9 S and coded for by the heavy strand has also been obtained.
摘要
对来自海拉细胞线粒体裂解物沉降图谱中多核糖体区域的含聚腺苷酸（poly(A)）RNA进行变性条件下的聚丙烯酰胺凝胶电泳和沉降分析，结果显示存在一种离散的RNA组分，其在天然状态下的沉降常数约为7S。根据天然和变性条件下的沉降行为以及聚腺苷酸含量，估计该组分的分子量约为9×10⁴ 。RNA-DNA杂交实验表明，该组分由线粒体DNA的轻链编码。同时也获得了存在一种沉降约为9S且由重链编码的含聚腺苷酸RNA组分的证据。
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