HeLa细胞异质性核RNA中大小不同的聚腺苷酸序列的差异代谢
Differential metabolism of large and small poly(A) sequences in the heterogeneous nuclear RNA of HeLa cells.
作者信息
Nakazato H, Edmonds M, Kopp D W
出版信息
Proc Natl Acad Sci U S A. 1974 Jan;71(1):200-4. doi: 10.1073/pnas.71.1.200.
Abstract
The heterogeneous RNA of the HeLa cell nucleus contains short internal poly(A) sequences which, in contrast to the longer poly(A) sequences at the 3' ends, are not found in messenger RNA of the cytoplasm. A distinct origin for each of these homologous sequences is evident when the effects of actinomycin D and 3'-deoxyadenosine on their biosynthesis are compared. The shorter poly(A) appears to be transcribed, while the longer one does not. A kinetic analysis of the metabolism of each type of poly(A) sequence within different size classes of HnRNA after treatment of the cells with 3'-deoxyadenosine reveals distinctive distribution and metabolism of these sequences and also provides insight into mechanisms proposed for the generation of messenger RNA from these large RNA molecules in the nucleus.
摘要
海拉细胞核的不均一RNA含有短的内部多聚腺苷酸序列，与3'端较长的多聚腺苷酸序列不同，这些序列在细胞质的信使RNA中未被发现。当比较放线菌素D和3'-脱氧腺苷对其生物合成的影响时，这些同源序列中每一个的独特起源是明显的。较短的多聚腺苷酸似乎是被转录的，而较长的则不是。在用3'-脱氧腺苷处理细胞后，对不同大小类别的核内不均一RNA中每种多聚腺苷酸序列的代谢进行动力学分析，揭示了这些序列独特的分布和代谢情况，也为从细胞核中的这些大RNA分子产生信使RNA所提出的机制提供了见解。
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