采用放射免疫分析法和酶分析法对人血清多巴胺-β-羟化酶水平进行比较。
Comparison of human serum dopamine-beta-hydroxylase levels by radioimmunoassay and enzymatic assay.
作者信息
Rush R A, Thomas P E, Nagatsu T, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1974 Mar;71(3):872-4. doi: 10.1073/pnas.71.3.872.
Abstract
Human serum dopamine-beta-hydroxylase (3,4-dihydroxyphenylethylamine, ascorbate:oxygen oxidoreductase, EC 1.14.17.1) has been measured in a normal adult population by both enzymatic assay and by radioimmunoassay. The levels of enzymatic activity varied over a very wide range from 0.6 units/ml to 85 units/ml. In contrast, the amount of total dopamine-beta-hydroxylase protein present in these samples, as measured by radioimmunoassay, varied only over a 3-fold range: from 11.7 mug/ml to 34.4 mug/ml. From an analysis of the purified enzyme, the percentage of serum dopamine-beta-hydroxylase that was catalytically active was estimated to be small and variable.
摘要
已通过酶促测定法和放射免疫测定法对正常成年人群中的人血清多巴胺-β-羟化酶（3,4-二羟基苯乙胺，抗坏血酸：氧氧化还原酶，EC 1.14.17.1）进行了测量。酶活性水平在非常宽的范围内变化，从0.6单位/毫升到85单位/毫升。相比之下，通过放射免疫测定法测量，这些样品中存在的总多巴胺-β-羟化酶蛋白量仅在3倍范围内变化：从11.7微克/毫升到34.4微克/毫升。通过对纯化酶的分析，估计具有催化活性的血清多巴胺-β-羟化酶百分比很小且可变。
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