Dunnette J, Weinshilboum R
Am J Hum Genet. 1976 Mar;28(2):155-66.
An antibody against human adrenal dopamine beta-hydroxylase (DBH) was used to quantitate immunoreactive DBH protein in human serum by an immunoprecipitation technique. A significant correlation was found between DBH enzyme activity and immunoreactive DBH protein in randomly selected serum samples (r = 0.94; N = 38; p less than .001). Studies of sera from obligate heterozygotes and individuals homozygous for the allele responsible for very low serum DBH enzymatic activity were compatible with a genetically mediated decrease in the quantity of circulating DBH protein in these subjects.
采用一种抗人肾上腺多巴胺β-羟化酶（DBH）的抗体，通过免疫沉淀技术对人血清中的免疫反应性DBH蛋白进行定量分析。在随机选取的血清样本中，发现DBH酶活性与免疫反应性DBH蛋白之间存在显著相关性（r = 0.94；N = 38；p < 0.001）。对血清DBH酶活性极低的等位基因杂合子和纯合子个体的血清研究表明，这些受试者循环中DBH蛋白数量的减少是由基因介导的。
