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来自团藻的一种糖蛋白性诱导剂的纯化及部分特性分析

Purification and partial characterization of a glycoprotein sexual inducer from Volvox carteri.

作者信息

Kochert G, Yates I

出版信息

Proc Natl Acad Sci U S A. 1974 Apr;71(4):1211-4. doi: 10.1073/pnas.71.4.1211.

DOI:10.1073/pnas.71.4.1211
PMID:4524633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC388194/
Abstract

A method is described for the isolation and purification of the sexual inducer produced by male colonies of the colonial green alga Volvox carteri. The procedure yields a highly purified preparation of the inducer. The biologically active principal is demonstrated to be a glycoprotein with a molecular weight of 32,000.

摘要

本文描述了一种分离和纯化由群体绿藻卡特氏团藻雄性群体产生的性诱导剂的方法。该程序产生了高度纯化的诱导剂制剂。已证明具有生物活性的主要成分是一种分子量为32,000的糖蛋白。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/11cb4bfb6401/pnas00057-0207-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/48144954a505/pnas00057-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/95118413bcb1/pnas00057-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/11cb4bfb6401/pnas00057-0207-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/48144954a505/pnas00057-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/95118413bcb1/pnas00057-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c64/388194/11cb4bfb6401/pnas00057-0207-c.jpg

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1
Purification and partial characterization of a glycoprotein sexual inducer from Volvox carteri.来自团藻的一种糖蛋白性诱导剂的纯化及部分特性分析
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引用本文的文献

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Development. 2018 Apr 9;145(7):dev162537. doi: 10.1242/dev.162537.
2
Evolution of sexes from an ancestral mating-type specification pathway.从祖先交配型决定途径进化而来的性别。
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3
A Preliminary Genetic Investigation of VOLVOX CARTERI.卡特藻的初步遗传研究。

本文引用的文献

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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
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EQUILIBRIUM ULTRACENTRIFUGATION OF DILUTE SOLUTIONS.稀溶液的平衡超速离心法
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Sexual differentiation in Volvox aureus.金黄色团藻的性别分化
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Sexual Interaction in Heterothallic Strains of Closterium peracerosum-strigosum-littorale: Partial Characterization of a Male-Specific Sexual Pheromone, Protoplast-Releasing Substance.纤细新月藻-条纹新月藻-滨海新月藻异宗配合菌株中的性相互作用:雄性特异性性信息素——原生质体释放物质的部分特性
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L-Glutamic acid as a mediator of sexual morphogenesis in Volvox capensis.L-谷氨酸作为衣藻性形态发生的介质。
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High resolution acrylamide gel electrophoresis of histones.组蛋白的高分辨率丙烯酰胺凝胶电泳
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Differentiation of reproductive cells in Volvox carteri.团藻生殖细胞的分化
J Protozool. 1968 Aug;15(3):438-52. doi: 10.1111/j.1550-7408.1968.tb02154.x.
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Sites of synthesis of chloroplast membrane polypeptides in Chlamydomonas reinhardi y-1.莱茵衣藻y-1中叶绿体膜多肽的合成位点
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