在卡特氏团藻（Volvox carteri f. nagariensis Iyengar）中启动有性形态发生的激素的纯化与特性分析
Purification and characterization of the hormone initiating sexual morphogenesis in Volvox carteri f. nagariensis Iyengar.
作者信息
Starr R C, Jaenicke L
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1050-4. doi: 10.1073/pnas.71.4.1050.
Abstract
In Volvox carteri f. nagariensis male spheroids secrete into the medium a sexual hormone which controls the initiation of the developmental pathway leading to the formation of sexual embryos. In the absence of the hormone asexual embryos are formed. Analysis of the highly purified hormone indicates that it is a glycoprotein of 27,500-30,000 daltons, composed of one peptide chain, which has a typical composition except for a relatively low content of tryptophan. The glycosidic moiety, which accounts for about 45% of the weight of the molecule, consists of pentoses, hexoses, and amino hexoses. In the bioassay the highly purified hormone stimulates 100% formation of sexual embryos at a concentration of 10(-10) g/liter and 14.4% sexual embryos at 10(-11) g/liter (3 x 10(-16) M). The activity is remarkably thermostable and resistant to different denaturing procedures.
摘要
在多细胞绿藻（Volvox carteri f. nagariensis）中，雄性球体向培养基中分泌一种性激素，该激素控制着导致有性胚胎形成的发育途径的启动。在没有这种激素的情况下，会形成无性胚胎。对高度纯化的激素进行分析表明，它是一种分子量为27,500 - 30,000道尔顿的糖蛋白，由一条肽链组成，除色氨酸含量相对较低外，具有典型的组成。糖苷部分约占分子重量的45%，由戊糖、己糖和氨基己糖组成。在生物测定中，高度纯化的激素在浓度为10^(-10)克/升时刺激100%的有性胚胎形成，在10^(-11)克/升（3×10^(-16) M）时刺激14.4%的有性胚胎形成。该活性具有显著的热稳定性，并且对不同的变性程序具有抗性。
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本文引用的文献
1
Sex attractant in a brown alga: chemical structure.性引诱剂在褐藻中：化学结构。
Science. 1971 Feb 26;171(3973):815-7. doi: 10.1126/science.171.3973.815.
2
A method for determining the sedimentation behavior of enzymes: application to protein mixtures.一种测定酶沉降行为的方法：应用于蛋白质混合物
J Biol Chem. 1961 May;236:1372-9.
3
Production, isolation, and characterization of sirenin.交配素的制备、分离与特性鉴定
Biochemistry. 1966 Jul;5(7):2147-52. doi: 10.1021/bi00871a001.
4
Sexual differentiation in Volvox aureus.金黄色团藻的性别分化
J Protozool. 1966 May;13(2):239-55. doi: 10.1111/j.1550-7408.1966.tb01901.x.
5
The gel-filtration behaviour of proteins related to their molecular weights over a wide range.蛋白质的凝胶过滤行为与其在很宽范围内的分子量相关。
Biochem J. 1965 Sep;96(3):595-606. doi: 10.1042/bj0960595.
6
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
7
Differentiation of reproductive cells in Volvox carteri.团藻生殖细胞的分化
J Protozool. 1968 Aug;15(3):438-52. doi: 10.1111/j.1550-7408.1968.tb02154.x.
8
Size and charge isomer separation and estimation of molecular weights of proteins by disc gel electrophoresis.通过圆盘凝胶电泳进行蛋白质的大小和电荷异构体分离及分子量估计。
Arch Biochem Biophys. 1968 Jul;126(1):155-64. doi: 10.1016/0003-9861(68)90569-9.
9
Sexual hormones of Achlya and other fungi.
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10
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