Wiche G, Zomzely-Neurath C, Blume A J
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1446-50. doi: 10.1073/pnas.71.4.1446.
Polyribosomes were isolated from a clonal line of mouse neuroblastoma grown in culture. In a heterologous in vitro system containing rat brain components, these polyribosomes were shown to direct the synthesis of neuroblastoma tubulin. Identification of the tubulin synthesized in vitro was achieved by coelectrophoresis with native neuroblastoma tubulin on sodium dodecyl sulfate polyacrylamide gels, immunoprecipitation, and demonstration of specific aggregation. Tubulin accounted for 2% of the total proteins synthesized. This in vitro protein synthesizing system offers a model for studying possible translational control mechanisms regulating the synthesis of proteins involved in nerve cell function.
从培养的小鼠神经母细胞瘤克隆系中分离出多核糖体。在含有大鼠脑成分的异源体外系统中，这些多核糖体被证明可指导神经母细胞瘤微管蛋白的合成。通过在十二烷基硫酸钠聚丙烯酰胺凝胶上与天然神经母细胞瘤微管蛋白进行共电泳、免疫沉淀以及特异性聚集的证明，实现了对体外合成的微管蛋白的鉴定。微管蛋白占合成的总蛋白的2%。这个体外蛋白质合成系统为研究调节神经细胞功能相关蛋白质合成的可能翻译控制机制提供了一个模型。
