Jorgensen A O, Heywood S M
Proc Natl Acad Sci U S A. 1974 Nov;71(11):4278-82. doi: 10.1073/pnas.71.11.4278.
A protein-synthesizing system has been established in vitro, in which tubulin is synthesized on polysomes isolated from brain and leg muscle of embryonic chick. The tubulin synthesized in vitro is characterized by (i) its electrophoretic mobility on sodium dodecyl sulfate-acrylamide gels (brain: 55,000 daltons; leg muscle: 53,000 daltons), and (ii) its ability to function as a microtubular subunit, as judged by its specific ability to participate in at least two polymerization and depolymerization steps in the microtubule assembly system in vitro.
已在体外建立了一个蛋白质合成系统，其中微管蛋白是在从胚胎期小鸡的脑和腿部肌肉分离出的多核糖体上合成的。体外合成的微管蛋白具有以下特点：（i）其在十二烷基硫酸钠-丙烯酰胺凝胶上的电泳迁移率（脑：55,000道尔顿；腿部肌肉：53,000道尔顿），以及（ii）其作为微管亚基发挥功能的能力，这是根据其在体外微管组装系统中参与至少两个聚合和解聚步骤的特定能力来判断的。
