Evans D I, O'Rouke C, Jones P M
J Clin Pathol. 1974 Mar;27(3):226-30. doi: 10.1136/jcp.27.3.226.
The Cytocentrifuge enables a satisfactory cytological preparation to be made when the cells in the cerebrospinal fluid are normal in number or only slightly increased. A technique has been developed and the results of its use are described in 114 consecutive samples from 50 children with acute leukaemia, with and without involvement of the central nervous system. Analysis of the results showed that 30% of the samples with a normal cell count contained leukaemic cells when examined by Cytocentrifuge. Only 74% of the samples with raised counts were found to contain leukaemic cells. Diagnosis of leukaemic meningitis based on changes in the cell count alone in cerebrospinal fluid is clearly unreliable, and the Cytocentrifuge enables a more precise assessment of the cerebrospinal fluid to be made. It also appears that changes in the levels of protein and glucose in the cerebrospinal fluid of leukaemic patients are not directly related to the presence of leukaemic cells but are the results of changes in the cell count from whatever cause. There appears to be no value in measuring protein and glucose levels when monitoring the cerebrospinal fluid in acute leukaemia for evidence of early involvement of the central nervous system.
当脑脊液中的细胞数量正常或仅略有增加时，细胞离心涂片法能制备出令人满意的细胞学标本。现已开发出一种技术，并在来自50例急性白血病患儿的114份连续样本中描述了其应用结果，这些患儿伴有或不伴有中枢神经系统受累。结果分析表明，细胞离心涂片法检查时，细胞计数正常的样本中有30%含有白血病细胞。细胞计数升高的样本中只有74%被发现含有白血病细胞。仅根据脑脊液细胞计数的变化来诊断白血病性脑膜炎显然不可靠，而细胞离心涂片法能对脑脊液进行更精确的评估。似乎白血病患者脑脊液中蛋白质和葡萄糖水平的变化与白血病细胞的存在没有直接关系，而是细胞计数因任何原因发生变化的结果。在监测急性白血病患者的脑脊液以寻找中枢神经系统早期受累证据时，测量蛋白质和葡萄糖水平似乎没有价值。
