一种含胰高血糖素肽的分离：胰高血糖素原可能片段的一级结构
Isolation of a glucagon-containing peptide: primary structure of a possible fragment of proglucagon.
作者信息
Tager H S, Steiner D F
出版信息
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2321-5. doi: 10.1073/pnas.70.8.2321.
Abstract
The heterogeneity of crystalline bovine (ox)/porcine glucagon has been examined by gel filtration and ion-exchange chromatography. A strongly basic peptide that reacted well with antibodies to bovine/porcine glucagon was isolated and its primary structure was determined. The amino-acid sequence of the NH(2)-terminal 29 residues of the 4500-dalton peptide is identical with that of intact bovine or porcine glucagon. The remaining eight residues at its COOH-terminus are Lys-Arg-Asn-Asn-Lys-Asn-Ile-Ala. Small amounts of other glucagon-immunoreactive peptides having molecular weights ranging from 3700 to 9000 were also detected in crystals of bovine/porcine glucagon. We propose that the 37-residue peptide is a fragment of bovine or porcine proglucagon.
摘要
通过凝胶过滤和离子交换色谱法研究了结晶牛（牛）/猪胰高血糖素的异质性。分离出一种与牛/猪胰高血糖素抗体反应良好的强碱性肽，并确定了其一级结构。4500道尔顿肽的NH(2)-末端29个残基的氨基酸序列与完整的牛或猪胰高血糖素相同。其COOH-末端的其余八个残基是Lys-Arg-Asn-Asn-Lys-Asn-Ile-Ala。在牛/猪胰高血糖素晶体中也检测到少量分子量范围为3700至9000的其他胰高血糖素免疫反应性肽。我们认为37个残基的肽是牛或猪胰高血糖素原的片段。
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