一种可溶性超活性胰岛素形式。
A soluble super-active form of insulin.
作者信息
Oka T, Topper Y J
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1630-3. doi: 10.1073/pnas.71.5.1630.
Abstract
Insulin-like material can be extracted from insulin-Sepharose. This soluble material elicits super-insulin responses from mammary epithelial cells. It stimulates the accumulation of alpha-aminoisobutyric acid by virgin mouse mammary cells, which are unresponsive to insulin itself. It exerts a greater stimulatory effect on mammary cells from pregnant mice than insulin. It also is more efficacious than insulin as a stimulant of DNA synthesis and the combined activities of glucose-6-phosphate dehydrogenase (EC 1.1.1.49) and phosphogluconate dehydrogenase (EC 1.1.1.43). Its specific biological activity on these cells is more than five times greater than that of insulin.
摘要
胰岛素样物质可从胰岛素-琼脂糖中提取。这种可溶性物质能引发乳腺上皮细胞的超胰岛素反应。它能刺激未对胰岛素本身产生反应的处女鼠乳腺细胞积累α-氨基异丁酸。它对怀孕小鼠的乳腺细胞的刺激作用比胰岛素更大。作为DNA合成以及葡萄糖-6-磷酸脱氢酶（EC 1.1.1.49）和磷酸葡萄糖酸脱氢酶（EC 1.1.1.43）联合活性的刺激剂，它也比胰岛素更有效。它对这些细胞的特定生物学活性比胰岛素高五倍以上。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Cultivation of adult mouse mammary gland in hormone-enriched synthetic medium.在富含激素的合成培养基中培养成年小鼠乳腺。
Science. 1957 Oct 25;126(3278):842-3. doi: 10.1126/science.126.3278.842-a.
3
Increase in activity of glucose 6-phosphate dehydrogenase in mouse mammary tissue cultured in insulin.在胰岛素培养的小鼠乳腺组织中，葡萄糖6-磷酸脱氢酶活性增加。
Biochem J. 1969 Jun;113(1):175-82. doi: 10.1042/bj1130175.
4
Insulin-sepharose and the dynamics of insulin action.胰岛素琼脂糖凝胶及胰岛素作用动力学
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2066-8. doi: 10.1073/pnas.68.9.2066.
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Interaction of insulin with the cell membrane: the primary action of insulin.胰岛素与细胞膜的相互作用：胰岛素的主要作用。
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