Patterson D, Kao F T, Puck T T
Proc Natl Acad Sci U S A. 1974 May;71(5):2057-61. doi: 10.1073/pnas.71.5.2057.
Studies are presented on the biochemical genetics of 30 adenine-requiring mutants of the Chinese hamster ovary cell which were induced by mutagenesis and selected by the BrdU-visible light technique. Representative experiments conducted with these mutants include: hybridization with each other; hybridization with normal human cells; nutritional analysis; biochemical analysis with radioactively labeled intermediates; and measurement of reversion frequencies to wild-type phenotype occurring spontaneously and under the influence of selected mutagens. All mutants behave as if having point mutations. These experiments provide information relevant to the determination of dominant-recessive relationships, resolution into different complementation classes, localization of the human chromosomes which carry human genes required by the individual mutants, determination of the point of metabolic block for different mutants, and elucidation of the nature of the underlying DNA changes. These experiments illustrate the range of biochemical-genetic studies now possible with such a family of somatic mammalian cell mutants in vitro. Possible application to problems of human genetic disease are indicated.
本文介绍了对中国仓鼠卵巢细胞的30个腺嘌呤需求型突变体进行的生化遗传学研究。这些突变体通过诱变产生，并采用BrdU-可见光技术进行筛选。对这些突变体进行的代表性实验包括：相互杂交；与正常人细胞杂交；营养分析；用放射性标记中间体进行生化分析；以及测量自发产生和在选定诱变剂影响下回复到野生型表型的频率。所有突变体的行为都像是发生了点突变。这些实验为确定显性-隐性关系、解析为不同的互补类、定位携带各个突变体所需人类基因的人类染色体、确定不同突变体的代谢阻断点以及阐明潜在DNA变化的性质提供了相关信息。这些实验说明了利用这样一组体外培养的体细胞哺乳动物细胞突变体进行生化遗传学研究的范围。文中还指出了其在人类遗传疾病问题上的可能应用。
