塞姆利基森林病毒膜中刺突糖蛋白的定位

Proc Natl Acad Sci U S A. 1974 Oct;71(10):3988-92. doi: 10.1073/pnas.71.10.3988.

Labeling experiments with formyl-[(35)S]-methionyl sulfone methylphosphate and crosslinking studies with dimethylsuberimidate suggest that the spike glycoproteins of Semliki Forest virus extend through the viral membrane into close contact with the nucleocapsid. Based on this finding, we present a mechanism for the formation of virus-specific patches in the host cell plasma membrane during virus assembly.

用甲酰基 - [³⁵S] - 蛋氨酰砜甲基磷酸酯进行的标记实验以及用亚胺基二甲酯进行的交联研究表明，辛德毕斯病毒的刺突糖蛋白穿过病毒膜并与核衣壳紧密接触。基于这一发现，我们提出了一种在病毒组装过程中宿主细胞质膜上形成病毒特异性斑块的机制。