来自噬菌体T4的溶菌酶的三维结构。
The three dimensional structure of the lysozyme from bacteriophage T4.
作者信息
Matthews B W, Remington S J
出版信息
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4178-82. doi: 10.1073/pnas.71.10.4178.
Abstract
The three dimensional structure of the lysozyme from bacteriophage T4 has been determined from a 2.5 A resolution electron density map. About 60% of the molecule is in a helical conformation and there is one region consisting of antiparallel beta-structure. The polypeptide backbone folds into two distinct lobes linked in part by a long helix. In the region between the two lobes, there is a cleft which deepens into a hole or cavity, about 6-8 A in diameter, extending from one side of the molecule to the other. This opening is closed off by side chains which extend to within 3-5 A of each other. A number of mutant lysozymes in which residues in the vicinity of the opening are modified have markedly reduced catalytic activity, suggesting that this region of the molecule may be catalytically important. The three dimensional structure of T4 phage lysozyme is quite different from that of hen egg-white lysozyme although it is not clear at this time whether or not the mechanisms of catalysis of the respective enzymes are related.
摘要
已从分辨率为2.5埃的电子密度图确定了噬菌体T4溶菌酶的三维结构。该分子约60%呈螺旋构象，有一个区域由反平行β结构组成。多肽主链折叠成两个不同的叶，部分由一个长螺旋连接。在两个叶之间的区域，有一个裂缝，该裂缝加深形成一个直径约6 - 8埃的孔或腔，从分子的一侧延伸到另一侧。这个开口被相互延伸至彼此3 - 5埃范围内的侧链封闭。许多在开口附近残基被修饰的突变溶菌酶催化活性显著降低，这表明分子的这个区域可能在催化方面很重要。噬菌体T4溶菌酶的三维结构与鸡蛋清溶菌酶的三维结构有很大不同，尽管目前尚不清楚各自酶的催化机制是否相关。
相似文献
1
The three dimensional structure of the lysozyme from bacteriophage T4.来自噬菌体T4的溶菌酶的三维结构。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4178-82. doi: 10.1073/pnas.71.10.4178.
2
3
Crystal structure of the lysozyme from bacteriophage lambda and its relationship with V and C-type lysozymes.噬菌体λ溶菌酶的晶体结构及其与V型和C型溶菌酶的关系。
J Mol Biol. 1998 Feb 13;276(1):151-64. doi: 10.1006/jmbi.1997.1499.
4
Structure of the lysozyme from bacteriophage T4: an electron density map at 2.4 A resolution.噬菌体T4溶菌酶的结构：分辨率为2.4埃的电子密度图。
J Mol Biol. 1978 Jan 5;118(1):81-98. doi: 10.1016/0022-2836(78)90245-0.
5
A covalent enzyme-substrate adduct in a mutant hen egg white lysozyme (D52E).突变型鸡卵清溶菌酶（D52E）中的共价酶-底物加合物。
J Biol Chem. 1997 Aug 8;272(32):19976-81. doi: 10.1074/jbc.272.32.19976.
6
[Conformational rearrangements of bacteriophage T4 lysozyme during its binding to the inhibitor].[噬菌体T4溶菌酶与抑制剂结合过程中的构象重排]
Biokhimiia. 1979 Oct;44(10):1864-76.
7
Comparison of goose-type, chicken-type, and phage-type lysozymes illustrates the changes that occur in both amino acid sequence and three-dimensional structure during evolution.鹅型、鸡型和噬菌体型溶菌酶的比较说明了在进化过程中氨基酸序列和三维结构所发生的变化。
J Mol Evol. 1984;21(2):97-111. doi: 10.1007/BF02100084.
8
Lack of distant relationship between lysozyme Ch and hen egg white lysozyme: computer comparison studies.溶菌酶Ch与鸡蛋清溶菌酶之间缺乏远缘关系：计算机比较研究。
Experientia. 1981 Apr 15;37(4):339-40. doi: 10.1007/BF01959849.
9
Goose lysozyme structure: an evolutionary link between hen and bacteriophage lysozymes?鹅溶菌酶结构：母鸡与噬菌体溶菌酶之间的进化联系？
Nature. 1983 Jun 30;303(5920):828-31. doi: 10.1038/303828a0.
10
Clearing of suspensions of Micrococcus lysodeikticus catalysed by lysozymes from hen, goose, and turkey egg whites, human milk, and phage T4. Assessment of potential as signal generators for homogeneous enzyme immunoassays for urinary steroids.由来自母鸡、鹅和火鸡的蛋清、人乳中的溶菌酶以及噬菌体T4催化的溶壁微球菌悬浮液的清除。评估其作为尿甾体类物质均相酶免疫测定信号发生器的潜力。
J Immunoassay Immunochem. 2007;28(2):67-90. doi: 10.1080/15321810701209704.
引用本文的文献
1
Machine learning/molecular dynamic protein structure prediction approach to investigate the protein conformational ensemble.机器学习/分子动力学蛋白质结构预测方法研究蛋白质构象集合。
Sci Rep. 2022 Jun 15;12(1):10018. doi: 10.1038/s41598-022-13714-z.
2
Structure and Function of the Autolysin SagA in the Type IV Secretion System of .SagA 自溶素在 型 IV 分泌系统中的结构与功能
Mol Cells. 2021 Jul 31;44(7):517-528. doi: 10.14348/molcells.2021.0011.
3
Protein folding - seeing is deceiving.蛋白质折叠——眼见未必为实。
Protein Sci. 2021 Aug;30(8):1606-1616. doi: 10.1002/pro.4096. Epub 2021 May 7.
4
Crystal structure of bacteriophage T4 Spackle as determined by native SAD phasing.通过天然 SAD 相位测定法确定的噬菌体 T4 Spackle 的晶体结构。
Acta Crystallogr D Struct Biol. 2020 Sep 1;76(Pt 9):899-904. doi: 10.1107/S2059798320010979. Epub 2020 Aug 25.
5
Hydration-Induced Structural Changes in the Solid State of Protein: A SAXS/WAXS Study on Lysozyme.水合诱导的蛋白质固态结构变化：溶菌酶的小角 X 射线散射/广角 X 射线散射研究。
Mol Pharm. 2020 Sep 8;17(9):3246-3258. doi: 10.1021/acs.molpharmaceut.0c00351. Epub 2020 Aug 19.
6
Role of His101 in the Protein Folding/Unfolding of a Goose-Type Lysozyme from Ostrich (Struthio camelus) Egg White.组氨酸101在鸵鸟（鸵鸟属骆驼）蛋清中鹅型溶菌酶蛋白质折叠/去折叠过程中的作用
Protein J. 2016 Dec;35(6):416-423. doi: 10.1007/s10930-016-9687-5.
7
Single-Molecule Chemo-Mechanical Spectroscopy Provides Structural Identity of Folding Intermediates.单分子化学机械光谱法揭示折叠中间体的结构特征
Biophys J. 2016 Mar 29;110(6):1280-90. doi: 10.1016/j.bpj.2015.12.042.
8
Distributions of experimental protein structures on coarse-grained free energy landscapes.粗粒度自由能景观上实验蛋白质结构的分布。
J Chem Phys. 2015 Dec 28;143(24):243153. doi: 10.1063/1.4937940.
9
Biophysical highlights from 54 years of macromolecular crystallography.54 年来的大分子晶体学的生物物理亮点。
Biophys J. 2014 Feb 4;106(3):510-25. doi: 10.1016/j.bpj.2014.01.001.
10
Selective pressure causes an RNA virus to trade reproductive fitness for increased structural and thermal stability of a viral enzyme.选择压力导致 RNA 病毒为了增加病毒酶的结构和热稳定性而牺牲生殖适应性。
PLoS Genet. 2012;8(11):e1003102. doi: 10.1371/journal.pgen.1003102. Epub 2012 Nov 29.
本文引用的文献
1
BAGSHAPED MACROMOLECULES--A NEW OUTLOOK ON BACTERIAL CELL WALLS.袋状大分子——对细菌细胞壁的新视角
Adv Enzymol Relat Subj Biochem. 1964;26:193-232. doi: 10.1002/9780470122716.ch5.
2
Sequence similarities between hen egg-white and T4 phage lysozymes.鸡蛋清溶菌酶与T4噬菌体溶菌酶之间的序列相似性。
Nature. 1967 Aug 5;215(5101):621-2. doi: 10.1038/215621a0.
3
Three-dimensional structure of tosyl-alpha-chymotrypsin.甲苯磺酰-α-胰凝乳蛋白酶的三维结构。
Nature. 1967 May 13;214(5089):652-6. doi: 10.1038/214652a0.
4
Structure of hen egg-white lysozyme. A three-dimensional Fourier synthesis at 2 Angstrom resolution.鸡蛋清溶菌酶的结构。2埃分辨率下的三维傅里叶合成。
Nature. 1965 May 22;206(4986):757-61. doi: 10.1038/206757a0.
5
Role of a buried acid group in the mechanism of action of chymotrypsin.一个埋藏的酸性基团在胰凝乳蛋白酶作用机制中的作用。
Nature. 1969 Jan 25;221(5178):337-40. doi: 10.1038/221337a0.
6
Structure of subtilisin BPN' at 2.5 angström resolution.枯草杆菌蛋白酶BPN'在2.5埃分辨率下的结构
Nature. 1969 Jan 18;221(5177):235-42. doi: 10.1038/221235a0.
7
The matching of physical models to three-dimensional electron-density maps: a simple optical device.物理模型与三维电子密度图的匹配：一种简单的光学装置。
J Mol Biol. 1968 Oct 14;37(1):225-30. doi: 10.1016/0022-2836(68)90085-5.
8
Frameshift mutation in the lysozyme gene of bacteriophage T4: demonstration of the insertion of five bases, and a summary of in vivo codons and lysozyme activities.噬菌体T4溶菌酶基因中的移码突变：五个碱基插入的证明以及体内密码子和溶菌酶活性的总结
J Mol Biol. 1970 Dec 14;54(2):219-46. doi: 10.1016/0022-2836(70)90428-6.
9
Frameshift mutations and the genetic code. This paper is dedicated to Professor Theodosius Dobzhansky on the occasion of his 66th birthday.移码突变与遗传密码。本文谨献给西奥多修斯·杜布赞斯基教授，以庆祝他66岁生日。
Cold Spring Harb Symp Quant Biol. 1966;31:77-84. doi: 10.1101/sqb.1966.031.01.014.
10
The structure of thermolysin: an electron density map at 2-3 A resolution.嗜热菌蛋白酶的结构：分辨率为2 - 3埃的电子密度图。
J Mol Biol. 1972 Oct 14;70(3):701-24. doi: 10.1016/0022-2836(72)90569-4.
Zotero 插件