在冠瘿瘤中未检测到根癌土壤杆菌DNA和PS8噬菌体DNA。
Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors.
作者信息
Chilton M D, Currier T C, Farrand S K, Bendich A J, Gordon M P, Nester E W
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3672-6. doi: 10.1073/pnas.71.9.3672.
Abstract
Renaturation kinetics of labeled Agrobacterium tumefaciens DNA are not influenced by addition of 10(4)-fold excess of crown gall tumor DNA. Reconstruction experiments demonstrated that 0.01% added bacterial DNA produces a detectable increase in rate of renaturation of labeled DNA. Crown gall tumor DNA therefore cannot contain as much as 0.01% A. tumefaciens DNA (one entire bacterial genome per three diploid tumor cells). By the same technique, PS8 bacteriophage DNA is not detected in crown gall tumor DNA under conditions that allow detection of 0.0007-0.001% added phage DNA. Crown gall tumor DNA cannot contain as much as one entire phage genome per diploid tumor cell. The presence of a small fraction of either genome (less than 5%), even if present as multiple copies, would escape detection by this method.
摘要
标记的根癌土壤杆菌DNA的复性动力学不受添加10⁴倍过量的冠瘿瘤DNA的影响。重建实验表明，添加0.01%的细菌DNA会使标记DNA的复性速率产生可检测到的增加。因此，冠瘿瘤DNA所含的根癌土壤杆菌DNA不可能多达0.01%（每三个二倍体肿瘤细胞中含有一个完整的细菌基因组）。通过相同的技术，在能够检测到添加0.0007 - 0.001%噬菌体DNA的条件下，未在冠瘿瘤DNA中检测到PS8噬菌体DNA。冠瘿瘤DNA不可能每个二倍体肿瘤细胞中含有多达一个完整的噬菌体基因组。即使以多拷贝形式存在，任何一种基因组的一小部分（小于5%）的存在都可能无法通过这种方法检测到。
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