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酵母苯丙氨酸转运RNA两种晶体形式的分子结构保守性

Conservation of the molecular structure of yeast phenylalanine transfer RNA in two crystal forms.

作者信息

Klug A, Robertus J D, Ladner J E, Brown R S, Finch J T

出版信息

Proc Natl Acad Sci U S A. 1974 Sep;71(9):3711-5. doi: 10.1073/pnas.71.9.3711.

DOI:10.1073/pnas.71.9.3711
PMID:4530331
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC433846/
Abstract

A comparison is made between the electron density maps of the monoclinic and orthorhombic crystal forms of yeast tRNA(Phe) which have been obtained respectively by ourselves and by another group. It is concluded that the molecular structures are essentially the same in both crystals, although the models derived from the maps are not the same. The relation between the two molecular packings is discussed, and it is suggested that the intermolecular contact in the orthorhombic form which is not present in the monoclinic form, may arise through base pairing of the anticodons of neighboring molecules.

摘要

对我们自己和另一组分别获得的酵母苯丙氨酸转运核糖核酸(tRNA(Phe))单斜晶系和正交晶系晶体形式的电子密度图进行了比较。得出的结论是,尽管从这些图推导出来的模型不同,但两种晶体中的分子结构基本相同。讨论了两种分子堆积之间的关系,并提出正交晶系形式中不存在于单斜晶系形式中的分子间接触,可能是通过相邻分子反密码子的碱基配对产生的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d5/433846/f4eae61e27db/pnas00072-0400-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d5/433846/66c8d8e0ccda/pnas00072-0400-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d5/433846/f4eae61e27db/pnas00072-0400-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d5/433846/66c8d8e0ccda/pnas00072-0400-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41d5/433846/f4eae61e27db/pnas00072-0400-b.jpg

相似文献

1
Conservation of the molecular structure of yeast phenylalanine transfer RNA in two crystal forms.酵母苯丙氨酸转运RNA两种晶体形式的分子结构保守性
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3711-5. doi: 10.1073/pnas.71.9.3711.
2
The molecular structure of yeast phenylalanine transfer RNA in monoclinic crystals.单斜晶体中酵母苯丙氨酸转移核糖核酸的分子结构
Proc Natl Acad Sci U S A. 1974 May;71(5):2146-50. doi: 10.1073/pnas.71.5.2146.
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Yeast phenylalanine transfer RNA: atomic coordinates and torsion angles.酵母苯丙氨酸转运核糖核酸:原子坐标与扭转角
Nucleic Acids Res. 1975 Dec;2(12):2329-41. doi: 10.1093/nar/2.12.2329.
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Restrained refinement of two crystalline forms of yeast aspartic acid and phenylalanine transfer RNA crystals.酵母天冬氨酸和苯丙氨酸转移RNA晶体两种晶型的约束精修
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A comparison of the fluorescence of the Y base of yeast tRNA-Phe in solution and in crystals.
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X-ray diffraction patterns of transfer RNA consistent with the presence of short parallel helices in the molecule.转运RNA的X射线衍射图谱与该分子中存在短平行螺旋结构相一致。
Science. 1971 Jun 18;172(3989):1234-7. doi: 10.1126/science.172.3989.1234.
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Atomic coordinates and molecular conformation of yeast phenylalanyl tRNA. An independent investigation.酵母苯丙氨酰tRNA的原子坐标和分子构象。一项独立研究。
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High-resolution x-ray diffraction patterns of crystalline transfer RNA that show helical regions.显示螺旋区域的结晶转移核糖核酸的高分辨率X射线衍射图谱。
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Orientation of double-helical segments in crystals of yeast phenylalanine transfer RNA.酵母苯丙氨酸转移核糖核酸晶体中双螺旋片段的取向
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The three-dimensional structure of yeast phenylalanine transfer RNA: shape of the molecule at 5.5-A resolution.酵母苯丙氨酸转运核糖核酸的三维结构:5.5埃分辨率下分子的形状
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3746-50. doi: 10.1073/pnas.69.12.3746.

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本文引用的文献

1
Correlation between three-dimensional structure and chemical reactivity of transfer RNA.转运核糖核酸的三维结构与化学反应性之间的相关性
Nucleic Acids Res. 1974 Jul;1(7):927-32. doi: 10.1093/nar/1.7.927.
2
High-resolution x-ray diffraction patterns of crystalline transfer RNA that show helical regions.显示螺旋区域的结晶转移核糖核酸的高分辨率X射线衍射图谱。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):841-5. doi: 10.1073/pnas.68.4.841.
3
Orientation of double-helical segments in crystals of yeast phenylalanine transfer RNA.酵母苯丙氨酸转移核糖核酸晶体中双螺旋片段的取向
转运RNA(tRNA)与核糖体在A位点和P位点的相互作用。
EMBO J. 1995 Oct 2;14(19):4872-82. doi: 10.1002/j.1460-2075.1995.tb00168.x.
4
Interaction of unfolded tRNA with the 3'-terminal region of E. coli 16S ribosomal RNA.未折叠的tRNA与大肠杆菌16S核糖体RNA 3'末端区域的相互作用。
Nucleic Acids Res. 1985 Sep 11;13(17):6283-98. doi: 10.1093/nar/13.17.6283.
5
Defects in mitochondrial protein synthesis and respiratory chain activity segregate with the tRNA(Leu(UUR)) mutation associated with mitochondrial myopathy, encephalopathy, lactic acidosis, and strokelike episodes.线粒体蛋白合成和呼吸链活性缺陷与与线粒体肌病、脑病、乳酸性酸中毒和类卒中发作相关的tRNA(Leu(UUR))突变共分离。
Mol Cell Biol. 1992 Feb;12(2):480-90. doi: 10.1128/mcb.12.2.480-490.1992.
6
Respiration-deficient cells are caused by a single point mutation in the mitochondrial tRNA-Leu (UUR) gene in mitochondrial myopathy, encephalopathy, lactic acidosis, and strokelike episodes (MELAS).呼吸缺陷细胞是由线粒体肌病、脑病、乳酸酸中毒和卒中样发作(MELAS)中线粒体tRNA-亮氨酸(UUR)基因的单点突变引起的。
Am J Hum Genet. 1991 Sep;49(3):590-9.
7
Atomic co-ordinates for yeast phenylalanine tRNA.酵母苯丙氨酸转运核糖核酸的原子坐标。
Nucleic Acids Res. 1975 Sep;2(9):1629-37. doi: 10.1093/nar/2.9.1629.
8
The binding of polyamines and of ethidium bromide to tRNA.多胺及溴化乙锭与转运核糖核酸(tRNA)的结合
Nucleic Acids Res. 1975 Jul;2(7):1005-22. doi: 10.1093/nar/2.7.1005.
J Mol Biol. 1973 Oct 25;80(2):255-63. doi: 10.1016/0022-2836(73)90171-x.
4
Structure of yeast phenylalanine tRNA at 3 A resolution.酵母苯丙氨酸tRNA在3埃分辨率下的结构
Nature. 1974 Aug 16;250(467):546-51. doi: 10.1038/250546a0.
5
Three-dimensional tertiary structure of yeast phenylalanine transfer RNA.酵母苯丙氨酸转移核糖核酸的三维三级结构。
Science. 1974 Aug 2;185(4149):435-40. doi: 10.1126/science.185.4149.435.
6
Three-dimensional structure of yeast phenylalanine transfer RNA at 3.0angstroms resolution.酵母苯丙氨酸转运核糖核酸在3.0埃分辨率下的三维结构。
Nature. 1974 Mar 1;248(5443):20-4. doi: 10.1038/248020a0.
7
Unit cell transormations in yeast phenylalanine transfer RNA crystals.酵母苯丙氨酸转运RNA晶体中的晶胞转变
J Mol Biol. 1973 Apr 5;75(2):429-32. doi: 10.1016/0022-2836(73)90032-6.
8
High-resolution x-ray diffraction studies on a pure species of transfer RNA.
J Mol Biol. 1972 Dec 14;72(1):99-101. doi: 10.1016/0022-2836(72)90071-x.
9
The three-dimensional structure of yeast phenylalanine transfer RNA: shape of the molecule at 5.5-A resolution.酵母苯丙氨酸转运核糖核酸的三维结构:5.5埃分辨率下分子的形状
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3746-50. doi: 10.1073/pnas.69.12.3746.
10
The molecular structure of yeast phenylalanine transfer RNA in monoclinic crystals.单斜晶体中酵母苯丙氨酸转移核糖核酸的分子结构
Proc Natl Acad Sci U S A. 1974 May;71(5):2146-50. doi: 10.1073/pnas.71.5.2146.