与含蛋白A的葡萄球菌黏附的淋巴细胞的密度梯度分离
Density gradient separation of lymphoid cells adhering to protein-A-containing staphylococci.
作者信息
Ghetie V, Nilsson K, Sjöquist J
出版信息
Proc Natl Acad Sci U S A. 1974 Dec;71(12):4831-5. doi: 10.1073/pnas.71.12.4831.
A cell separation technique was designed based on the interaction between cell-surface-bound IgG and protein A of Staphylococcus aureus. The density of lymphoid cells coated with IgG antibodies against one of the surface markers was increased by adherence of staphylococci. Cells with adhering bacteria were separated from cells without bacteria by density gradient centrifugation in 11.5% sodium metrizoate. Bacteria were removed from the lymphoid cells by lysostaphin digestion. The purity of separated cells was approximately 95% even when the proportion of a specific cell population was below 10% in the initial mixture. The viability and the ability of cells to multiply in vitro were not significantly impaired by the fractionating procedure. The technique can generally be applied for cell separation, provided antibodies of the IgG class against specific surface markers are available.
基于细胞表面结合的IgG与金黄色葡萄球菌蛋白A之间的相互作用，设计了一种细胞分离技术。通过葡萄球菌的黏附，针对一种表面标志物的IgG抗体包被的淋巴细胞密度增加。通过在11.5%的甲泛影酸钠中进行密度梯度离心，将黏附有细菌的细胞与没有细菌的细胞分离。通过溶葡萄球菌素消化从淋巴细胞中去除细菌。即使特定细胞群体在初始混合物中的比例低于10%，分离细胞的纯度仍约为95%。分级分离过程对细胞的活力和体外增殖能力没有明显损害。只要有针对特定表面标志物的IgG类抗体，该技术一般可用于细胞分离。
Zotero 插件