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与绵羊β-促脂素羧基末端五十个氨基酸相对应的具有高脂肪分解活性的五十肽的合成。

Synthesis of a pentekontapeptide with high lipolytic activity corresponding to the carboxyl-terminal fifty amino acids of ovine beta-lipotropin.

作者信息

Yamashiro D, Li C H

出版信息

Proc Natl Acad Sci U S A. 1974 Dec;71(12):4945-9. doi: 10.1073/pnas.71.12.4945.

DOI:10.1073/pnas.71.12.4945
PMID:4531028
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC434016/
Abstract

The synthesis of a peptide, composed of fifty amino-acid residues, corresponding to positions 42-91 in ovine beta-lipotropin has been accomplished by the solidphase method. The preformed symmetrical anhydride and active ester coupling methods were used exclusively. The synthetic product was purified by gel filtration, carboxymethylcellulose chromatography, and partition chromatography on Sephadex G-50. Its lipolytic activity in isolated rabbit fat cells was about six times that of beta-lipotropin on a weight basis.

摘要

已通过固相法合成了一种由五十个氨基酸残基组成的肽，该肽对应于羊β-促脂素的42-91位。仅使用了预先形成的对称酸酐和活性酯偶联方法。合成产物通过凝胶过滤、羧甲基纤维素色谱法以及在Sephadex G-50上的分配色谱法进行纯化。以重量计，其在分离的兔脂肪细胞中的脂解活性约为β-促脂素的六倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1110/434016/04a919109625/pnas00075-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1110/434016/04a919109625/pnas00075-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1110/434016/04a919109625/pnas00075-0310-a.jpg

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SOLID-PHASE PEPTIDE SYNTHESIS. 3. AN IMPROVED SYNTHESIS OF BRADYKININ.固相肽合成。3. 缓激肽的改进合成法。
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METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.分离脂肪细胞的代谢。I. 激素对葡萄糖代谢和脂肪分解的影响。
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